Babij P, Booth F W
Department of Physiology and Cell Biology, Medical School, University of Texas Health Science Center, Houston 77225.
Am J Physiol. 1988 May;254(5 Pt 1):C651-6. doi: 10.1152/ajpcell.1988.254.5.C651.
Specific complementary DNA (cDNA) hybridization probes were used to estimate the levels of alpha-actin and cytochrome c mRNAs and also 18S rRNA in three models of skeletal muscle atrophy. After 7 days of hindlimb suspension, or immobilization, or denervation, protein content decreased 26-32% in all muscles studied except suspended fast-twitch muscle, which lost only half as much protein. alpha-Actin mRNA content decreased 51-66% and cytochrome c mRNA content decreased 42-61% in slow- and fast-twitch muscles in all three models of atrophy. However, total RNA content did not show similar directional changes; RNA content decreased 27-44% in suspended and immobilized muscle but was unchanged in denervated fast-twitch muscle. The results were interpreted to suggest that loss of weight-bearing function of skeletal muscle is a major factor affecting the levels of alpha-actin and cytochrome c mRNAs during muscle atrophy.
使用特异性互补DNA（cDNA）杂交探针来估计三种骨骼肌萎缩模型中α-肌动蛋白和细胞色素c mRNA以及18S rRNA的水平。在后肢悬吊、固定或去神经支配7天后，除悬吊的快肌外，所有研究的肌肉中蛋白质含量下降了26%-32%，而悬吊的快肌仅损失了一半的蛋白质。在所有三种萎缩模型的慢肌和快肌中，α-肌动蛋白mRNA含量下降了51%-66%，细胞色素c mRNA含量下降了42%-61%。然而，总RNA含量并未呈现类似的方向性变化；悬吊和固定肌肉中的RNA含量下降了27%-44%，而去神经支配的快肌中的RNA含量未发生变化。这些结果被解释为表明骨骼肌负重功能的丧失是影响肌肉萎缩期间α-肌动蛋白和细胞色素c mRNA水平的主要因素。
