猫淋巴细胞性胆管炎中肝祖细胞及其微环境激活的免疫组织化学评估。
Immunohistochemical evaluation of the activation of hepatic progenitor cells and their niche in feline lymphocytic cholangitis.
作者信息
Otte Corma Ma, Valtolina Chiara, Vreman Sandra, Hubers Siobhan, van Wolferen Monique E, Favier Robert P, Rothuizen Jan, Penning Louis C
机构信息
1 Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
2 Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
出版信息
J Feline Med Surg. 2018 Jan;20(1):30-37. doi: 10.1177/1098612X17699723. Epub 2017 Mar 28.
Objectives The aim of the study was to compare the hepatic progenitor cell niche in healthy feline livers and the liver tissue of cats with lymphocytic cholangitis. Methods Immunohistochemical stainings for vimentin, laminin, beta (β)-catenin and Notch1 intracellular domain (NICD) were used on formalin-fixed liver biopsies from affected (n = 12) and unaffected cats (n = 2). Results All immunohistochemical markers used were expressed in more cells, or more intensely, in the liver tissue of cats with lymphocytic cholangitis than in the liver tissue of unaffected cats. Conclusions and relevance Enhanced expression of vimentin, laminin, cytoplasmic/nuclear β-catenin and NICD in liver biopsies from cats with lymphocytic cholangitis indicates that the hepatic progenitor cell (HPC) niche is remodelled and activated. HPCs might provide insights into new regenerative treatment options for lymphocytic cholangitis in cats in the future.
目的 本研究旨在比较健康猫肝脏和淋巴细胞性胆管炎猫肝脏组织中的肝祖细胞龛。方法 对来自患病猫（n = 12）和未患病猫（n = 2）的福尔马林固定肝脏活检组织进行波形蛋白、层粘连蛋白、β（β）-连环蛋白和Notch1细胞内结构域（NICD）的免疫组织化学染色。结果 与未患病猫的肝脏组织相比，淋巴细胞性胆管炎猫的肝脏组织中所有使用的免疫组织化学标志物在更多细胞中表达，或表达更强。结论及相关性 淋巴细胞性胆管炎猫肝脏活检组织中波形蛋白、层粘连蛋白、细胞质/细胞核β-连环蛋白和NICD的表达增强表明肝祖细胞（HPC）龛被重塑和激活。未来，HPCs可能为猫淋巴细胞性胆管炎的新再生治疗选择提供见解。
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