Zhang Peili, Zuo Zhigui, Shang Wenjing, Wu Aihua, Bi Ruichun, Wu Jianbo, Li Shaotang, Sun Xuecheng, Jiang Lei
1 Central Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
2 Department of Colorectal Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Tumour Biol. 2017 Mar;39(3):1010428317694546. doi: 10.1177/1010428317694546.
Circular RNA, a class of non-coding RNA, is a new group of RNAs and is related to tumorigenesis. Circular RNAs are suggested to be ideal candidate biomarkers with potential diagnostic and therapeutic implications. However, little is known about their expression in human colorectal cancer. In our study, differentially expressed circular RNAs were detected using circular RNA array in paired tumor and adjacent non-tumorous tissues from six colorectal cancer patients. Expression levels of selected circular RNAs (hsa_circRNA_103809 and hsa_circRNA_104700) were measured by real-time polymerase chain reaction in 170 paired colorectal cancer samples for validation. Statistical analyses were conducted to investigate the association between hsa_circRNA_103809 and hsa_circRNA_104700 expression levels and respective patient clinicopathological features. Receiver operating characteristic curve was constructed to evaluate the diagnostic values. Our results indicated that there were 125 downregulated and 76 upregulated circular RNAs in colorectal cancer tissues compared with normal tissues. We also first demonstrated that the expression levels of hsa_circRNA_103809 ( p < 0.0001) and hsa_circRNA_104700 ( p = 0.0003) were significantly lower in colorectal cancer than in normal tissues. The expression level of hsa_circRNA_103809 was significantly correlated with lymph node metastasis ( p = 0.021) and tumor-node-metastasis stage ( p = 0.011), and the expression level of hsa_circRNA_104700 was significantly correlated with distal metastasis ( p = 0.036). The area under receiver operating characteristic curves of hsa_circRNA_103809 and hsa_circRNA_104700 were 0.699 ( p < 0.0001) and 0.616 ( p < 0.0001), respectively. In conclusion, these results suggest that hsa_circRNA_103809 and hsa_circRNA_104700 may be potentially involved in the development of colorectal cancer and serve as potential biomarkers for the diagnosis of colorectal cancer.
环状RNA是一类非编码RNA，是一组新的RNA，与肿瘤发生有关。环状RNA被认为是具有潜在诊断和治疗意义的理想候选生物标志物。然而，人们对它们在人类结直肠癌中的表达知之甚少。在我们的研究中，使用环状RNA阵列在6例结直肠癌患者的配对肿瘤组织和相邻非肿瘤组织中检测到差异表达的环状RNA。通过实时聚合酶链反应在170对结直肠癌样本中测量所选环状RNA（hsa_circRNA_103809和hsa_circRNA_104700）的表达水平以进行验证。进行统计分析以研究hsa_circRNA_103809和hsa_circRNA_104700表达水平与各自患者临床病理特征之间的关联。构建受试者工作特征曲线以评估诊断价值。我们的结果表明，与正常组织相比，结直肠癌组织中有125个环状RNA下调，76个环状RNA上调。我们还首次证明，hsa_circRNA_103809（p <0.0001）和hsa_circRNA_104700（p = 0.0003）在结直肠癌中的表达水平明显低于正常组织。hsa_circRNA_103809的表达水平与淋巴结转移（p = 0.021）和肿瘤-淋巴结-转移分期（p = 0.011）显著相关，hsa_circRNA_104700的表达水平与远处转移（p = 0.036）显著相关。hsa_circRNA_103809和hsa_circRNA_104700的受试者工作特征曲线下面积分别为0.699（p <0.0001）和0.616（p <0.0001）。总之，这些结果表明，hsa_circRNA_103809和hsa_circRNA_104700可能潜在参与结直肠癌的发生发展，并可作为结直肠癌诊断的潜在生物标志物。
