用苯基 -N -叔丁基硝酮捕获半胱氨酸硫自由基。
Spin trapping the cysteine thiyl radical with phenyl-N-t-butylnitrone.
作者信息
Graceffa P
机构信息
Boston Biomedical Research Institute, Department of Muscle Research, MA 02114.
出版信息
Biochim Biophys Acta. 1988 May 18;954(2):227-30. doi: 10.1016/0167-4838(88)90076-3.
The cysteine thiyl radical has been detected in a variety of biological systems by means of the ESR spectrum of the adduct between the radical and nitrone spin traps. 5,5-Dimethyl-1-pyroline N-oxide (DMPO) is the spin trap of choice in these studies for several reasons. However, we show here that the adduct between the cysteine thiyl radical and phenyl-N-t-butylnitrone (PBN) spin trap can be observed under certain oxidizing conditions where the adduct with DMPO is not detected. This suggests the use of PBN in searching for the thiyl radical under such conditions.
通过自由基与硝酮自旋捕集剂之间加合物的电子自旋共振光谱，已在多种生物系统中检测到半胱氨酸硫基自由基。5,5-二甲基-1-吡咯啉N-氧化物（DMPO）是这些研究中的首选自旋捕集剂，原因有几个。然而，我们在此表明，在某些氧化条件下，可以观察到半胱氨酸硫基自由基与苯基-N-叔丁基硝酮（PBN）自旋捕集剂之间的加合物，而此时检测不到与DMPO的加合物。这表明在这种条件下可使用PBN来寻找硫基自由基。
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