1 The Central Biomedical Laboratory, Aino University School of Health Science , Ibaraki City, Osaka, Japan .
2 Department of Physical Therapy, Aino University School of Health Science , Ibaraki City, Osaka, Japan .
J Neurotrauma. 2017 Nov 1;34(21):3003-3011. doi: 10.1089/neu.2016.4841. Epub 2017 Jun 29.
The effects of multiple injection of bone marrow mononuclear cells (BMNCs) on spinal cord injury (SCI) were compared with those of single injection in rats. BMNCs separated by density-gradient centrifugation from a bone marrow perfusate were injected three times (once weekly) through the cerebrospinal fluid (CSF) via the fourth ventricle, and the locomotor improvement and tissue recovery, including axonal regeneration, were compared with those of single injection. While the single-injection group showed a steep elevation of the Basso-Beattie-Bresnahan (BBB) score 1 week after transplantation, the multiple-injection group maintained a similar steep elevation for 2 weeks after transplantation, and the BBB scores of the multiple-injection group remained thereafter at a level approximately 2-3 points higher than those of the single-injection group until the end of the experiment. There were significant differences between the single- and multiple-injection groups at 3, 4, and 8 weeks after transplantation. The difference in BBB scores at 8 weeks after transplantation suggested that there was a marked difference in the quality of locomotor behaviors between the single-and multiple-injection groups at this stage. An extensive outgrowth of regenerating axons through the astrocyte-devoid areas and a marked reduction of cavity formation were found in both the single- and multiple-injection groups. There were, however, no significant differences in the density of regenerating axons or volumes of cavities between the single- and multiple-injection groups. These results showed that although tissue recoveries were similar between single and multiple injection, the multiple injection of BMNCs was more beneficial for locomotor improvement than single injection for the treatment of SCI. Considering the technically simple and low-cost procedures for the preparation and injection of BMNCs, multiple injection of BMNCs by lumbar puncture has an advantage over single injection on clinical application.
将骨髓单个核细胞（BMNCs）多次注射与单次注射治疗大鼠脊髓损伤（SCI）的效果进行了比较。通过第四脑室从骨髓灌流液中经脑脊液（CSF）三次（每周一次）注入分离的 BMNCs，并比较运动改善和组织恢复，包括轴突再生。单次注射组在移植后 1 周 BBB 评分急剧升高，而多次注射组在移植后 2 周内保持相似的急剧升高，此后多次注射组的 BBB 评分一直保持在比单次注射组高约 2-3 分的水平，直至实验结束。移植后 3、4 和 8 周时，单次和多次注射组之间存在显著差异。移植后 8 周 BBB 评分的差异表明，在该阶段，单次和多次注射组的运动行为质量存在明显差异。在单次和多次注射组中，再生轴突穿过星形胶质细胞缺失区域广泛生长，腔形成明显减少。然而，在再生轴突密度或腔体积方面，单次和多次注射组之间没有显著差异。这些结果表明，尽管单次和多次注射的组织恢复相似，但与单次注射相比，多次注射 BMNCs 更有利于 SCI 的运动改善。考虑到 BMNCs 的制备和注射技术简单且成本低，经腰椎穿刺多次注射 BMNCs 在临床应用上优于单次注射。
