肿瘤表面刷检在检测头颈癌中人乳头瘤病毒DNA方面的敏感性。

Sensitivity of tumor surface brushings to detect human papilloma virus DNA in head and neck cancer.

作者信息

Kofler Barbara, Borena Wegene, Manzl Claudia, Dudas Jozsef, Wegscheider Anne-Sophie, Jansen-Dürr Pidder, Schartinger Volker, Riechelmann Herbert

机构信息

Department of Otorhinolaryngology, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.

Division of Virology, Department of Hygiene, Microbiology, Social Medicine, Medical University of Innsbruck, Peter-Mayr-Strasse 4b, 6020 Innsbruck, Austria.

出版信息

Oral Oncol. 2017 Apr;67:103-108. doi: 10.1016/j.oraloncology.2017.02.013. Epub 2017 Feb 23.

DOI:10.1016/j.oraloncology.2017.02.013

PMID:28351563

Abstract

OBJECTIVE

Human papilloma virus (HPV) induced head and neck squamous cell carcinoma (HNSCC) represents a distinct tumor subset. We questioned how accurately a brushing from the tumor surface detects HPV in patients with HNSCC.

MATERIALS AND METHODS

Brushings from the tumor surface were compared with HPV DNA isolation from formalin-fixed and paraffin-embedded (FFPE) tumor biopsies, which served as the reference standard. In both matrices, HPV DNA was detected using a commercially available test kit. In addition, p16 was assessed in tumor biopsies by immunohistochemistry (IHC). The tumors were considered p16 positive if 70% or more of cancer cells expressed p16.

RESULTS

93 patients with HNSCC were included. Sensitivity and specificity of the brush test were 83% (95%CI: 67-92%) and 85% (95%CI: 72-93%). Results of p16 IHC were concordant with FFPE samples DNA determinations in 73/93 patients. In 53 patients (57%) the tumor was located in the oropharynx and in 40 patients (43%) the tumor was located in the non-oropharynx region. Sensitivity and specificity of the brush test in patients with oropharyngeal cancer was higher with 86% (95%CI: 70-95%) and 89% (95%CI: 65-99%).

CONCLUSION

Superficial brushes from the tumor surface may be used to identify HPV positive HNSCC.

摘要

目的

人乳头瘤病毒（HPV）诱发的头颈部鳞状细胞癌（HNSCC）是一种独特的肿瘤亚型。我们探讨了在HNSCC患者中，从肿瘤表面获取的刷片检测HPV的准确性如何。

材料与方法

将肿瘤表面的刷片与从福尔马林固定石蜡包埋（FFPE）肿瘤活检组织中分离的HPV DNA进行比较，后者作为参考标准。在这两种样本中，均使用市售检测试剂盒检测HPV DNA。此外，通过免疫组织化学（IHC）检测肿瘤活检组织中的p16。如果70%或更多癌细胞表达p16，则认为肿瘤为p16阳性。

结果

纳入93例HNSCC患者。刷检的敏感性和特异性分别为83%（95%CI：67-92%）和85%（95%CI：72-93%）。在73/93例患者中，p16 IHC结果与FFPE样本DNA检测结果一致。53例患者（57%）肿瘤位于口咽，40例患者（43%）肿瘤位于非口咽区域。口咽癌患者刷检的敏感性和特异性更高，分别为86%（95%CI：70-95%）和89%（95%CI：65-99%）。