Klimushina M V, Gumanova N G, Metelskaya V A
National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation.
National Research Center for Preventive Medicine of the Ministry of Healthcare of the Russian Federation, Moscow, 101990, Russian Federation.
Biochem Biophys Res Commun. 2017 May 6;486(3):824-826. doi: 10.1016/j.bbrc.2017.03.136. Epub 2017 Mar 27.
Analysis of serum proteome by antibody microarray is used to identify novel biomarkers and to study signaling pathways including protein phosphorylation and protein-protein interactions. Labeling of serum proteins is important for optimal performance of the antibody microarray. Proper choice of fluorescent label and optimal concentration of protein loaded on the microarray ensure good quality of imaging that can be reliably scanned and processed by the software. We have optimized direct serum protein labeling using fluorescent dye Arrayit Green 540 (Arrayit Corporation, USA) for antibody microarray. Optimized procedure produces high quality images that can be readily scanned and used for statistical analysis of protein composition of the serum.
通过抗体微阵列分析血清蛋白质组可用于鉴定新型生物标志物，并研究包括蛋白质磷酸化和蛋白质-蛋白质相互作用在内的信号通路。血清蛋白的标记对于抗体微阵列的最佳性能很重要。荧光标记的正确选择以及微阵列上加载的蛋白质的最佳浓度可确保成像质量良好，从而能够由软件进行可靠的扫描和处理。我们已经优化了使用荧光染料Arrayit Green 540（美国Arrayit公司）对血清蛋白进行直接标记的方法，以用于抗体微阵列。优化后的方法可产生高质量的图像，这些图像可轻松扫描并用于血清蛋白质组成的统计分析。
