Gumanova Nadezhda G, Zlobina Polina D, Bogdanova Natalya L, Brutyan Hakob A, Kalemberg Elena N, Metelskaya Victoria A, Davtyan Karapet V, Drapkina Oksana M
Department of Biochemistry, National Research Center for Preventive Medicine (NRCPM), Moscow, Russia.
Front Cardiovasc Med. 2023 May 24;10:1190051. doi: 10.3389/fcvm.2023.1190051. eCollection 2023.
Adenovirus (AdV) has been suggested to be involved in pathogenesis of atrial fibrillation (AF). We aimed to evaluate an association between AdV-specific immunoglobulins G in the serum (AdV-IgG) and AF. The present case-control study comprised two cohorts, including cohort 1 of patients with AF and cohort 2 of asymptomatic subjects. Initially, two groups, MA and MB, were selected from the cohorts 1 and 2, respectively, for serum proteome profiling using an antibody microarray to identify possible relevant protein targets. The data of microarray analysis indicated a possible overall increase in the total adenovirus signals in the group MA vs. group MB, suggesting potential relevance of adenoviral infection to AF. Then, the groups A (with AF) and B (control) were selected from the cohorts 1 and 2, respectively, to assay the presence and levels of AdV-IgG- by ELSA. The prevalence of AdV-IgG-positive status demonstrated a 2-fold increase in the group A (AF) compared with that in the group B (asymptomatic subjects); odds ratio 2.06 (95%CI: 1.11-3.84; = 0.02). The prevalence of obesity demonstrated an approximately 3-fold increase in AdV-IgG-positive patients of the group A compared with that in AdV-IgG-negative patients of the same group A (odds ratio 2.7; 95% CI: 1.02-7.1; = 0.04). Thus, AdV-IgG-positive reactivity was independently associated with AF, and AF was independently associated with BMI, indicating that adenoviral infection may be a possible etiological factor for AF.
腺病毒（AdV）被认为与心房颤动（AF）的发病机制有关。我们旨在评估血清中腺病毒特异性免疫球蛋白G（AdV-IgG）与AF之间的关联。本病例对照研究包括两个队列，即AF患者队列1和无症状受试者队列2。最初，分别从队列1和队列2中选择两组，MA组和MB组，使用抗体微阵列进行血清蛋白质组分析，以确定可能相关的蛋白质靶点。微阵列分析数据表明，MA组的腺病毒总信号可能比MB组总体增加，提示腺病毒感染与AF可能相关。然后，分别从队列1和队列2中选择A组（AF患者）和B组（对照组），通过酶联免疫吸附测定法（ELSA）检测AdV-IgG的存在和水平。AdV-IgG阳性状态的患病率在A组（AF患者）中比B组（无症状受试者）增加了2倍；优势比为2.06（95%置信区间：1.11 - 3.84；P = 0.02）。肥胖患病率在A组AdV-IgG阳性患者中比同一A组AdV-IgG阴性患者增加了约3倍（优势比2.7；95%置信区间：1.02 - 7.1；P = 0.04）。因此，AdV-IgG阳性反应与AF独立相关，且AF与体重指数（BMI）独立相关，表明腺病毒感染可能是AF的一个可能病因。
