趋化因子受体CCR5和CXCR4可能在传染性法氏囊病病毒（IBDV）感染期间影响病毒复制。

Chemokine receptor CCR5 and CXCR4 might influence virus replication during IBDV infection.

作者信息

Ou Changbo, Wang Qiuxia, Yu Yan, Zhang Yanhong, Ma Jinyou, Kong Xianghui, Liu Xingyou

机构信息

Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; College of Life Science, Henan Normal University, Xinxiang 453007, Henan, China; College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.

Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China; College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang 453003, Henan, China.

出版信息

Microb Pathog. 2017 Jun;107:122-128. doi: 10.1016/j.micpath.2017.03.031. Epub 2017 Mar 27.

DOI:10.1016/j.micpath.2017.03.031

PMID:28351707

Abstract

Both CCR5 and CXCR4 are important chemokine receptors and take vital role in migration, development and distribution of T cells, however, whether they will influence the process of T cell infiltration into bursa of Fabricius during infectious bursal disease virus (IBDV) infection is unclear. In the current study, CCR5 and CXCR4 antagonists, Maraviroc and AMD3100, were administrated into chickens inoculated with IBDV, and the gene levels of IBDV VP2, CCR5, CXCR4 and related cytokines were determined by real-time PCR. The results showed that large number of T cells began to migrate into the bursae on Day 3 post infection with IBDV and the mRNA of chemokine receptors CCR5 and CXCR4 began to increase on Day 1. Moreover, antagonist treatments have increased the VP2, CCR5 and CXCR4 gene transcriptions and influenced on the gene levels of IL-2, IL-6, IL-8, IFN-γ, TGF-β4, MHC-I and MDA5. In conclusion, the chemokine receptors CCR5 and CXCR4 might influence virus replication during IBDV infection and further study would focus on the interaction between chemokine receptors and their ligands.

摘要

CCR5和CXCR4均为重要的趋化因子受体，在T细胞的迁移、发育和分布中发挥着关键作用。然而，它们是否会在传染性法氏囊病病毒（IBDV）感染期间影响T细胞浸润法氏囊的过程尚不清楚。在本研究中，将CCR5和CXCR4拮抗剂马拉维若和AMD3100施用于接种IBDV的鸡，并通过实时PCR测定IBDV VP2、CCR5、CXCR4及相关细胞因子的基因水平。结果显示，感染IBDV后第3天大量T细胞开始迁移至法氏囊，趋化因子受体CCR5和CXCR4的mRNA在第1天开始增加。此外，拮抗剂处理增加了VP2、CCR5和CXCR4的基因转录，并影响了IL-2、IL-6、IL-8、IFN-γ、TGF-β4、MHC-I和MDA5的基因水平。总之，趋化因子受体CCR5和CXCR4可能在IBDV感染期间影响病毒复制，进一步的研究将聚焦于趋化因子受体与其配体之间的相互作用。