Ou Changbo, Wang Qiuxia, Zhang Yanhong, Kong Weili, Zhang Shouping, Yu Yan, Ma Jinyou, Liu Xingyou, Kong Xianghui
College of Life Science, Henan Normal University, Xinxiang, 453007, Henan, China.
Postdoctoral Research and Development Base, Henan Institute of Science and Technology, Xinxiang, 453003, Henan, China.
Virol J. 2017 May 10;14(1):93. doi: 10.1186/s12985-017-0757-x.
Infectious bursal disease virus (IBDV) infection causes immunosuppression in chickens and increases their susceptibility to secondary infections. To explore the interaction between host and IBDV, RNA-Seq was applied to analyse the transcriptional profiles of the responses of chickens' bursas of Fabricius in the early stage of IBDV infection.
The results displayed that a total of 15546 genes were identified in the chicken bursa libraries. Among the annotated genes, there were 2006 and 4668 differentially expressed genes in the infection group compared with the mock group on day 1 and day 3 post inoculation (1 and 3 dpi), respectively. Moreover, there were 676 common up-regulated and 83 common down-regulated genes in the bursae taken from the chickens infected with IBDV on both 1 and 3 dpi. Meanwhile, there were also some characteristic differentially expressed genes on 1 and 3 dpi. On day 1 after inoculation with IBDV, host responses mainly displayed immune response processes, while metabolic pathways played an important role on day three post infection. Six genes were confirmed by quantitative reverse transcription-PCR.
In conclusion, the differential gene expression profile demonstrated with RNA-Seq might offer a better understanding of the molecular interactions between host and IBDV during the early stage of infection.
传染性法氏囊病病毒（IBDV）感染可导致鸡免疫抑制，并增加其对继发感染的易感性。为探究宿主与IBDV之间的相互作用，应用RNA测序技术分析IBDV感染早期鸡法氏囊的转录谱。
结果显示，在鸡法氏囊文库中共鉴定出15546个基因。在注释基因中，接种后第1天（1 dpi）和第3天（3 dpi），感染组与 mock组相比，分别有2006个和4668个差异表达基因。此外，在1 dpi和3 dpi感染IBDV的鸡的法氏囊中，有676个共同上调基因和83个共同下调基因。同时，在1 dpi和3 dpi也有一些特征性差异表达基因。接种IBDV后第1天，宿主反应主要表现为免疫反应过程，而感染后第3天代谢途径起重要作用。通过定量逆转录PCR验证了6个基因。
总之，RNA测序显示的差异基因表达谱可能有助于更好地理解感染早期宿主与IBDV之间的分子相互作用。
