Young J M, Cheadle C, Foulke J S, Drohan W N, Sarver N
Molecular Biology Division, Rorer Biotechnology, Inc., Springfield, VA 22151.
Gene. 1988;62(2):171-85. doi: 10.1016/0378-1119(88)90556-2.
Epstein-Barr virus (EBV) replicons which include the genetic element oriP and a functional gene for Epstein-Barr nuclear antigen (EBNA-1) can be maintained episomally in a variety of mammalian cell lines [Yates et al., Nature 313 (1985) 812-815]. We have assessed the application of an EBV replicon for foreign gene expression. Two cDNAs, human interferon-gamma (IFN-gamma) and the extracellular domain of the human epidermal growth factor receptor (EGF-Rex), cloned in an EBV replicon, were efficiently expressed and the protein was secreted into the extracellular media. Expression in human embryonic 293 cells was approximately ten-fold higher than in CV-1 cells. The expression of the human protein is dependent upon the orientation of the IFN-gamma transcriptional cassette relative to the other genetic elements within the vector.
包含遗传元件oriP和爱泼斯坦-巴尔核抗原（EBNA-1）功能基因的爱泼斯坦-巴尔病毒（EBV）复制子可以在多种哺乳动物细胞系中以附加体形式维持存在[耶茨等人，《自然》313（1985）812 - 815]。我们评估了一种EBV复制子在异源基因表达中的应用。克隆到EBV复制子中的两个人类cDNA，即人γ干扰素（IFN-γ）和人表皮生长因子受体的胞外结构域（EGF-Rex），得到了高效表达，并且蛋白质分泌到了细胞外培养基中。在人胚胎293细胞中的表达比在CV-1细胞中高约十倍。人蛋白质的表达取决于IFN-γ转录盒相对于载体中其他遗传元件的方向。
