一种高度重复的鱼类核苷酸序列的克隆与特性分析
Cloning and characterization of a highly repetitive fish nucleotide sequence.
作者信息
Datta U, Dutta P, Mandal R K
机构信息
Department of Biochemistry, Bose Institute, Calcutta, India.
出版信息
Gene. 1988;62(2):331-6. doi: 10.1016/0378-1119(88)90570-7.
PMID:2835294
Abstract
We have cloned and sequenced a highly repetitive HindIII fragment of DNA from the common carp Cyprinus carpio. It represents a tandemly repeated sequence with a monomeric unit of 245 bp and comprises 8% of the fish genome. Higher units of this monomer appear as a ladder in Southern blots. The monomeric unit has been sequenced; it is A + T-rich with some direct and some inverse-repeat nucleotide clusters.
摘要
我们已经克隆并测序了鲤鱼（Cyprinus carpio）DNA的一个高度重复的HindIII片段。它代表了一个串联重复序列，单体单元为245 bp，占鱼类基因组的8%。在Southern杂交中，该单体的更高聚合单元呈现为阶梯状。单体单元已被测序；它富含A+T，含有一些直接和一些反向重复核苷酸簇。
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