孔鳐（板鳃亚纲，软骨鱼纲）基因组中由HindIII检测到的一种重复DNA的克隆与特性分析

Cloning and characterization of a repetitive DNA detected by HindIII in the genome of Raja montagui (Batoidea, Chondrichthyes).

作者信息

Rocco L, Stingo V, Bellitti M

机构信息

Department of Evolutionary and Comparative Biology, University of Naples, Federico II, Italy.

出版信息

Gene. 1996 Oct 17;176(1-2):185-9. doi: 10.1016/0378-1119(96)00244-2.

DOI:10.1016/0378-1119(96)00244-2

PMID:8918251

Abstract

A repetitive HindIII fragment of DNA from Raja montagui (Rajiformes) was cloned and sequenced for the first time in cartilaginous fishes. This element, which comprises approximately 5% of the whole genome of the spotted ray, is absent in long tandem arrays, being typical of satellite DNA. It appeared constituted by 311 AT-rich bp (61%). The clone was hybridized to the genomic DNA of species with varying phyletic distances, revealing a high degree of conservation.

摘要

首次在软骨鱼类中克隆并测序了来自蒙氏鳐（鳐形目）的一段重复性HindIII DNA片段。这种元件约占斑点鳐全基因组的5%，不存在于长串联阵列中，是卫星DNA的典型特征。它似乎由311个富含AT的碱基对（61%）组成。该克隆与不同系统发育距离物种的基因组DNA杂交，显示出高度的保守性。