Thabet Ahmed, Honscha Walther, Daugschies Arwid, Bangoura Berit
Institute of Parasitology, Faculty of Veterinary Medicine, Centre for Infectious Diseases, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany.
Institute of Pharmacy, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany.
Parasitol Res. 2017 May;116(5):1553-1559. doi: 10.1007/s00436-017-5432-z. Epub 2017 Mar 28.
Polyether ionophores are widely used to treat and control coccidiosis in chickens. Widespread use of anticoccidials resulted in worldwide resistance. Mechanisms of resistance development and expansion are complex and poorly understood. Relative proteomic quantification using LC-MS/MS was used to compare sensitive reference strains (Ref-1, Ref-2) with putatively resistant and moderately sensitive field strains (FS-R, FS-mS) of Eimeria tenella after isotopic labelling with tandem mass tags (TMT). Ninety-seven proteins were identified, and 25 of them were regulated. Actin was significantly upregulated in resistant strains in comparison with their sensitive counterparts. On the other hand, microneme protein (MIC4) was downregulated in resistant strains. Optimization of labelling E. tenella sporozoites by TMT might identify further proteins that play a role in the obvious complex mechanism leading to resistance against Monensin.
聚醚离子载体广泛用于治疗和控制鸡的球虫病。抗球虫药的广泛使用导致了全球范围内的耐药性。耐药性产生和扩散的机制复杂且了解甚少。使用串联质量标签（TMT）进行同位素标记后，采用液相色谱-串联质谱（LC-MS/MS）的相对蛋白质组定量方法，比较柔嫩艾美耳球虫的敏感参考菌株（Ref-1、Ref-2）与推定的耐药和中度敏感的田间菌株（FS-R、FS-mS）。共鉴定出97种蛋白质，其中25种有表达调控。与敏感菌株相比，耐药菌株中的肌动蛋白显著上调。另一方面，耐药菌株中的微线体蛋白（MIC4）表达下调。通过TMT优化对柔嫩艾美耳球虫子孢子的标记，可能会鉴定出更多在导致对莫能菌素耐药的明显复杂机制中起作用的蛋白质。
