Galiero Alessia, Turchi Barbara, Pedonese Francesca, Nuvoloni Roberta, Cantile Carlo, Colombani Giuseppe, Forzan Mario, Cerri Domenico, Bandecchi Patrizia, Fratini Filippo
Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, Pisa, Italy.
Azienda U.S.L. 2, Lucca, Italy.
Folia Microbiol (Praha). 2017 Nov;62(6):471-477. doi: 10.1007/s12223-017-0518-7. Epub 2017 Mar 28.
Mycobacterium avium paratuberculosis (Map) is a pathogen which causes a chronic progressive granulomatous enteritis known as paratuberculosis or Johne's disease and it primarily affects wild and domestic ruminants. The aim of this research was to examine a flock which consisted of 294 goats and was located in Garfagnana district (Tuscany, Italy) performing ELISA tests, culture and IS900 PCR assay; direct diagnostic methods were carried out not only on bulk tank milk and cheese samples but also on individual milk and tissue specimens collected from nine subjects positive to ELISA tests. Out of 294 animals, 20 goats (6.8%) were positive to ELISA surveys. Bulk tank milk samples were negative to culture and to PCR assay carried out on the DNA extracted directly from them, while, with respect to cheese, Map was detected by culture in 2/12 (16.66%) cheeses ripened for 3-7 days, and by PCR in 2/12 (16.66%) cheeses ripened for 3-7 days and in 3/12 (25%) cheeses ripened for 45 days. Regarding individual milk samples, Map was detected by culture in 2/9 (22.22%) specimens and by PCR in 5/9 (55.55%) samples. Furthermore, Map was isolated from the intestine in 9/9 (100%) animals, from the mesenteric lymph nodes in 8/9 (88.88%) subjects, from the liver in 4/9 (44.44%) goats, from the spleen in 5/9 (55.55%) animals, while Map DNA was found in all the tissue samples analyzed.The results demonstrated the presence of paratuberculosis in a goat flock located in Garfagnana district (Tuscany, Italy).
副结核分枝杆菌（Map）是一种病原体，可引发一种称为副结核病或约内氏病的慢性进行性肉芽肿性肠炎，主要感染野生和家养反刍动物。本研究的目的是对一群位于加尔法尼亚纳地区（意大利托斯卡纳）的294只山羊进行检测，采用酶联免疫吸附测定（ELISA）试验、培养及IS900聚合酶链反应（PCR）分析；不仅对储奶罐牛奶和奶酪样本进行直接诊断方法检测，还对从9例ELISA检测呈阳性的个体采集的牛奶和组织样本进行检测。在294只动物中，20只山羊（6.8%）ELISA检测呈阳性。储奶罐牛奶样本培养及对直接提取的DNA进行PCR分析均为阴性，而对于奶酪，在12份成熟3至7天的奶酪中，2份（16.66%）通过培养检测到Map，在12份成熟3至7天的奶酪中有2份（16.66%）以及在12份成熟45天的奶酪中有3份（25%）通过PCR检测到Map。关于个体牛奶样本，2份（22.22%）样本通过培养检测到Map，5份（55.55%）样本通过PCR检测到Map。此外，9只（100%）动物的肠道中分离出Map，8只（88.88%）个体的肠系膜淋巴结中分离出Map，4只（44.44%）山羊的肝脏中分离出Map，5只（55.55%）动物的脾脏中分离出Map，而在所有分析的组织样本中均发现了Map DNA。结果表明，位于加尔法尼亚纳地区（意大利托斯卡纳）的一群山羊中存在副结核病。
