SILAC-IodoTMT 用于评估细胞蛋白质组及其氧化还原状态。
SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status.
机构信息
Biomedical Research Center, University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
Department of Genome Integrity, Institute of Molecular Genetics of the Czech Academy of Science, Prague, Czech Republic.
出版信息
Methods Mol Biol. 2023;2603:259-268. doi: 10.1007/978-1-0716-2863-8_21.
Stable isotope labeling by amino acids in cell culture (SILAC) and iodoacetyl tandem mass tag (iodoTMT) are well-implemented mass spectrometry-based approaches for quantification of proteins and for site-mapping of cysteine modification. We describe here a combination of SILAC and iodoTMT to assess ongoing changes in the global proteome and cysteine modification levels using liquid chromatography separation coupled with high-resolution mass spectrometry (LC-MS/MS).
稳定同位素标记的氨基酸细胞培养（SILAC）和碘乙酰胺串联质量标签（iodoTMT）是基于质谱的定量蛋白质和半胱氨酸修饰位点映射的成熟方法。我们在这里描述了 SILAC 和 iodoTMT 的组合，用于使用液相色谱分离和高分辨率质谱（LC-MS/MS）评估全局蛋白质组和半胱氨酸修饰水平的持续变化。
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