制备外周血单个核细胞（PBMCs）作为精神疾病蛋白质组学研究的模型。

Preparation of Peripheral Blood Mononuclear Cells (PBMCs) as a Model for Proteomic Studies of Psychiatric Disorders.

作者信息

Rahmoune Hassan, Guest Paul C

机构信息

Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK.

Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.

出版信息

Adv Exp Med Biol. 2017;974:299-303. doi: 10.1007/978-3-319-52479-5_28.

DOI:10.1007/978-3-319-52479-5_28

PMID:28353249

Abstract

Peripheral blood mononuclear cells (PBMCs) have been used as a surrogate model of brain function in studies of psychiatric disorders, such as schizophrenia. This chapter describes the preparation of PBMCs from whole blood using a density gradient/cell culture protocol. This includes collection of the PBMC culture media and preparation of cell extracts in order to provide the raw materials for proteomic analyses.

摘要

外周血单核细胞（PBMCs）已被用作精神疾病（如精神分裂症）研究中脑功能的替代模型。本章介绍了使用密度梯度/细胞培养方案从全血中制备PBMCs的方法。这包括收集PBMC培养基和制备细胞提取物，以便为蛋白质组学分析提供原材料。