精神疾病蛋白质组学生物标志物研究的血液采样与制备程序

Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders.

作者信息

Guest Paul C, Rahmoune Hassan

机构信息

Department of Biochemistry and Tissue Biology, Laboratory of Neuroproteomics, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária ZeferinoVaz, 13083-862, Campinas, Brazil.

Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK.

出版信息

Adv Exp Med Biol. 2017;974:141-147. doi: 10.1007/978-3-319-52479-5_9.

DOI:10.1007/978-3-319-52479-5_9

PMID:28353230

Abstract

A major challenge in proteomic biomarker discovery and validation for psychiatric diseases is the inherent biological complexity underlying these conditions. There are also many technical issues which hinder this process such as the lack of standardization in sampling, processing and storage of bio-samples in preclinical and clinical settings. This chapter describes a reproducible procedure for sampling blood serum and plasma that is specifically designed for maximizing data quality output in two-dimensional gel electrophoresis, multiplex immunoassay and mass spectrometry profiling studies.

摘要

精神疾病蛋白质组学生物标志物的发现与验证面临的一个主要挑战是这些病症背后固有的生物学复杂性。还有许多技术问题阻碍着这一进程，例如在临床前和临床环境中生物样本的采样、处理和存储缺乏标准化。本章介绍了一种用于采集血清和血浆的可重复程序，该程序是专门为在二维凝胶电泳、多重免疫测定和质谱分析研究中最大化数据质量输出而设计的。