Chichocki T, Gonsior B, Höfert M, Jarczyk L, Raith B, Rokita E, Strzalkowski A, Sych M
Academy of Medicine, Krakow, Poland.
Histochemistry. 1988;89(1):99-104. doi: 10.1007/BF00496591.
The femoral bone from the 18-day pregnancy embryo and an rib cartilage of mature mice have been investigated using PIXE (proton induced X-ray emission) in combination with a proton microprobe on snap frozen cryosectioned material. The localization and the results of quantitative measurement of P, S, Cl, K, Ca, Fe and Zn have been correlated with the histochemical localization of inorganic deposits. It has been found that in calcifying and degenerating cartilage of the growth plate there is substantial loss of S; this element being indicative for sulphate groups of glycosaminoglycans. This change seems to be an important factor conditioning the process of mineralization. Zn is found in higher concentration in mineralized tissues, both in embryonal and mature cartilage as well as in the bone, and this suggests that Zn is also involved in the mineralization process. The mineralization of rib cartilage exceeds that of embryonal bone, and the Ca/P ratio is higher in the former than in the hydroxyapatite of the latter. The method described is a useful analytical tool especially for such types of studies in which elements are not easily redistributed by freezing, cutting and drying; e.g. in investigations of mineral deposits.
利用质子诱导X射线发射（PIXE）结合质子微探针，对妊娠18天胚胎的股骨和成熟小鼠的肋软骨进行了研究，材料为速冻冷冻切片。磷、硫、氯、钾、钙、铁和锌的定位及定量测量结果与无机沉积物的组织化学定位相关。研究发现，生长板钙化和退变的软骨中硫大量流失；该元素是糖胺聚糖硫酸基团的指示物。这种变化似乎是影响矿化过程的一个重要因素。在矿化组织中，锌的浓度较高，在胚胎软骨和成熟软骨以及骨骼中均如此，这表明锌也参与了矿化过程。肋软骨的矿化程度超过胚胎骨，前者的钙磷比高于后者的羟基磷灰石。所描述的方法是一种有用的分析工具，尤其适用于元素不易因冷冻、切割和干燥而重新分布的此类研究；例如在矿质沉积物的研究中。
