Cichocki T, Gonsior B, Höfert M, Jarczyk L, Raith B, Rokita E, Strzalkowski A, Sych M
Academy of Medicine, Kraków, Poland.
Acta Histochem. 1989;85(1):39-45. doi: 10.1016/s0065-1281(89)80095-9.
The tracheal cartilage of mature mice have been investigated using PIXE (proton induced X-ray emission) in combination with a proton microprobe on snap frozen cryosectioned material. The localization and quantitative measurements of P, S, Cl, K, Ca, Fe, and Zn concentrations as well as direct and indirect assessment of glycosaminoglycans by measurement of S content and measurement of bound colloidal iron at pH = 1.8 has been performed. Adjacent sections were stained with the Hale method in Müller modifications and the v. Koss method for sulphated mucins and inorganic deposits respectively. It has been found that hyaline cartilage in trachea contains mineral deposits and that P + Ca amounts up to 22% of cartilage dry mass. The Ca/P ratio approaches 2 what indicates hydroxyapatite type crystals. The cartilage contains substantial amounts of S reflecting the presence of sulphate groups. It was found that the cartilage binds also colloidal iron at low pH. There is a good correlation between places with high amount of bound colloidal iron assessed by PIXE and places showing strong Prussian Blue staining. The Fe/S ratio was, however, much lower in the cartilage than in other tissues what indicates that the colloidal iron method does not give quantitative results. There were no regions showing substantial decrease in Fe/S ratio which we found previously as typical for degenerating and calcifying growth plate cartilage. This may be connected with a relatively low degree of calcification degree of the tracheal cartilage.
利用质子诱导X射线发射（PIXE）技术结合质子微探针，对成熟小鼠的气管软骨进行了研究，材料为速冻冷冻切片。已对磷、硫、氯、钾、钙、铁和锌的浓度进行了定位和定量测量，并通过测量硫含量以及在pH = 1.8时测量结合的胶体铁，对糖胺聚糖进行了直接和间接评估。相邻切片分别用米勒改良的黑尔法和v.科斯法对硫酸化粘蛋白和无机沉积物进行染色。研究发现，气管中的透明软骨含有矿物质沉积物，磷和钙的含量高达软骨干质量的22%。钙磷比接近2，表明为羟基磷灰石型晶体。软骨含有大量的硫，反映出硫酸基团的存在。研究发现，软骨在低pH值下也能结合胶体铁。通过PIXE评估的结合大量胶体铁的部位与显示强普鲁士蓝染色的部位之间存在良好的相关性。然而，软骨中的铁硫比远低于其他组织，这表明胶体铁法无法给出定量结果。没有发现铁硫比大幅下降的区域，而我们之前发现这是退化和钙化生长板软骨的典型特征。这可能与气管软骨相对较低的钙化程度有关。
