与巨细胞病毒补体结合IgM抗体相关的补体介导的细胞溶解活性。

Complement-mediated cytolytic activity associated with cytomegalovirus complement-fixing IgM antibody.

作者信息

Booth J C, Mohammed H S

机构信息

Department of Virology, St. George's Hospital Medical School, University of London, England.

出版信息

J Med Virol. 1988 Mar;24(3):351-9. doi: 10.1002/jmv.1890240312.

DOI:10.1002/jmv.1890240312

PMID:2835430

Abstract

Cytomegalovirus-specific complement-fixing IgM (CMV CF-IgM) antibody was detected in 12/84 (14.3 percent) sera containing CMV-specific IgM. Higher titres were obtained by using a purified antigen preparation containing enveloped virus particles and membrane fragments when compared with a crude extract of infected cells, suggesting that the CF component of CMV IgM reacts predominantly with antigens on the surface of the infected cells. This also accounts for its complement-mediated cytolytic activity. Neither CMV-specific non-complement-fixing IgM nor CMV-specific IgG demonstrates cytolytic activity.

摘要

在84份含有巨细胞病毒特异性IgM的血清中，检测到12份（14.3%）血清存在巨细胞病毒特异性补体结合IgM（CMV CF-IgM）抗体。与感染细胞的粗提物相比，使用含有包膜病毒颗粒和膜片段的纯化抗体制剂可获得更高的滴度，这表明CMV IgM的补体结合成分主要与感染细胞表面的抗原发生反应。这也解释了其补体介导的细胞溶解活性。CMV特异性非补体结合IgM和CMV特异性IgG均未表现出细胞溶解活性。