类风湿因子在巨细胞病毒免疫球蛋白M抗体补体结合试验和间接血凝试验中的作用。
Role of rheumatoid factor in complement fixation and indirect hemagglutination tests for immunoglobulin M antibody to cytomegalovirus.
作者信息
Cremer N E, Hoffman M, Lennette E H
出版信息
J Clin Microbiol. 1978 Aug;8(2):160-5. doi: 10.1128/jcm.8.2.160-165.1978.
Absorption of immunoglobulin M (IgM)-rheumatoid factor (RF) from serum samples by reaction with insolubilized gamma globulin reduced the complement-fixing (CF) antibody titer to cytomegalovirus (CMV) antigen to less than 1:2 in the IgM fraction of some, but not all, sera. Thus, IgM-CF activity in some sera appeared to be due to specific IgM anti-CMV antibody and in other sera to complexes of IgM-RF with antiviral IgG antibody. Prozones were present in the CF tests on IgM fractions. Increasing the concentration of antigen from 2 to 4 U reduced the prozone titer by one or two double dilutions. This observation suggested that a competition for antigen may be operating at low dilutions of IgM antibody fractions. Removal of RF had little or no effect on the reaction of the IgM fraction of sera with CMV by the indirect hemagglutination test.
通过与不溶性γ球蛋白反应从血清样本中吸收免疫球蛋白M（IgM）类风湿因子（RF），可使某些（但并非所有）血清的IgM部分中针对巨细胞病毒（CMV）抗原的补体结合（CF）抗体效价降至1:2以下。因此，某些血清中的IgM-CF活性似乎是由于特异性IgM抗CMV抗体，而在其他血清中则是由于IgM-RF与抗病毒IgG抗体的复合物。在对IgM部分进行的CF试验中存在前带现象。将抗原浓度从2 U提高到4 U可使前带效价降低一或两个双倍稀释度。这一观察结果表明，在IgM抗体部分的低稀释度下可能存在抗原竞争。通过间接血凝试验去除RF对血清IgM部分与CMV的反应几乎没有影响。
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