Sherbet Gajanan V, Mulherkar Leela
Department of Zoology, University of Poona, Poona-7, India.
Wilhelm Roux Arch Entwickl Mech Org. 1965 Nov;155(6):701-708. doi: 10.1007/BF00574185.
Chick embryos at the primitive-streak stage were treatedin vitro with 0.002 and 0.008% follicle-stimulating hormone (FSH)(NIH-FSH-S1) for 24 hours. Post-nodal primitive-streak pieces 0.8 mm behind the node level were grafted into host chick embryos at the primitive-streak stage to assess the capacity of the grafted pieces to produce inductions.Control grafts from donors maintained under identical conditions were unable to cause induction; all of them were resorbed into the host embryo. The post-nodal pieces treated with FSH acquired capacity to induce neural tissue. The grafts seemed to induce foregut formation also. FSH appeared to have supported differentiation of the grafts into somites and mesenchyme.
处于原条期的鸡胚在体外分别用0.002%和0.008%的促卵泡激素（FSH）（NIH-FSH-S1）处理24小时。在节点水平后方0.8毫米处的节点后原条组织块被移植到处于原条期的宿主鸡胚中，以评估移植组织块产生诱导作用的能力。来自在相同条件下饲养的供体的对照移植组织块无法引起诱导；它们全部被宿主胚胎吸收。用FSH处理过的节点后组织块获得了诱导神经组织形成的能力。这些移植组织块似乎还能诱导前肠形成。FSH似乎支持了移植组织块分化为体节和间充质。
