Kuramitsu H K
Department of Microbiology-Immunology, Northwestern University Medical-Dental Schools, Chicago, Illinois 60611.
Mol Microbiol. 1987 Sep;1(2):229-32. doi: 10.1111/j.1365-2958.1987.tb00516.x.
The gtfB gene coding for glucosyltransferase-I (GTF-I) activity previously isolated from Streptococcus mutans GS-5 was insertionally inactivated with the newly constructed transposon MudE in an Escherichia coli background. Insertion of MudE into various regions of the gtfB gene led to inactivation of GTF-I activity. The altered gene was introduced back into S. mutans GS-5 by transformation and produced mutants defective in insoluble glucan synthesis as well as the ability to colonize smooth surfaces in the presence of sucrose. Therefore, the MudE transposon can be utilized to produce specific mutants in oral streptococci as well as in other transformable Gram-positive bacteria expressing an erythromycin-resistance marker.
先前从变形链球菌GS-5中分离出的编码葡糖基转移酶-I（GTF-I）活性的gtfB基因，在大肠杆菌背景下被新构建的转座子MudE插入失活。MudE插入gtfB基因的各个区域导致GTF-I活性失活。通过转化将改变后的基因重新导入变形链球菌GS-5，产生了在不溶性葡聚糖合成以及在蔗糖存在下在光滑表面定殖能力方面存在缺陷的突变体。因此，MudE转座子可用于在口腔链球菌以及其他表达红霉素抗性标记的可转化革兰氏阳性细菌中产生特定突变体。
