Yang Qiang, Cai Dongbo, Chen Wenshou, Chen Huiying, Luo Wei
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.
State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan, China.
Front Bioeng Biotechnol. 2022 Sep 9;10:1010931. doi: 10.3389/fbioe.2022.1010931. eCollection 2022.
Currently, industrial production of l-threonine (Thr) is based on direct fermentation with microorganisms such as , which has the characteristics of low cost and high productivity. In order to elucidate the key metabolic features of the synthesis pathway of Thr in to provide clues for metabolic regulation or engineering of the strain, this study was carried out on an l-threonine over-producing strain, in terms of analyses of metabolic flux, enzyme control and metabonomics. Since environmental disturbance and genetic modification are considered to be two important methods of metabolic analysis, addition of phosphate in the media and comparison of strains with different genotypes were selected as the two candidates due to their significant influence in the biosynthesis of Thr. Some important targets including key nodes, enzymes and biomarkers were identified, which may provide target sites for rational design through engineering the Thrproducing strain. Finally, metabolic regulation aimed at one biomarker identified in this study was set as an example, which confirms that combined metabolic analyses may guide to improve the production of threonine in .
目前，L-苏氨酸（Thr）的工业生产基于用诸如[具体微生物名称未给出]等微生物进行直接发酵，其具有成本低和生产率高的特点。为了阐明[具体微生物名称未给出]中Thr合成途径的关键代谢特征，为该菌株的代谢调控或工程改造提供线索，本研究针对一株L-苏氨酸高产菌株，从代谢通量分析、酶控制和代谢组学方面展开。由于环境干扰和基因改造被认为是代谢分析的两种重要方法，鉴于它们在Thr生物合成中有显著影响，选择在培养基中添加磷酸盐以及比较不同基因型的菌株作为两个候选因素。确定了一些重要靶点，包括关键节点、酶和生物标志物，这可能为通过改造Thr生产菌株进行合理设计提供靶点。最后，以针对本研究中确定的一种生物标志物的代谢调控为例，证实了联合代谢分析可指导提高[具体微生物名称未给出]中苏氨酸的产量。
