Park Juhwan, Lim Sooa
Department of Pharmaceutical Engineering, Hoseo University, Asan-si 31499, Chungnam, Republic of Korea.
Microorganisms. 2024 Aug 15;12(8):1681. doi: 10.3390/microorganisms12081681.
() has become industrially important in producing glutamic acid and lysine since its discovery and has been the subject of proteomics and central carbon metabolism studies. The proteome changes depending on environmental conditions, nutrient availability, and stressors. Post-translational modification (PTMs), such as phosphorylation, methylation, and glycosylation, alter the function and activity of proteins, allowing them to respond quickly to environmental changes. Proteomics techniques, such as mass spectrometry and two-dimensional gel electrophoresis, have enabled the study of proteomes, identification of proteins, and quantification of the expression levels. Understanding proteomes and central carbon metabolism in microorganisms provides insight into their physiology, ecology, and biotechnological applications, such as biofuels, pharmaceuticals, and industrial enzyme production. Several attempts have been made to create efficient production strains to increase productivity in several research fields, such as genomics and proteomics. In addition to amino acids, is used to produce vitamins, nucleotides, organic acids, and alcohols, expanding its industrial applications. Considerable information has been accumulated, but recent research has focused on proteomes and central carbon metabolism. The development of genetic engineering technologies, such as CRISPR-Cas9, has improved production efficiency by allowing precise manipulation of the metabolic pathways of . In addition, methods for designing new metabolic pathways and developing customized strains using synthetic biology technology are gradually expanding. This review is expected to enhance the understanding of and its industrial potential and help researchers identify research topics and design studies.
自被发现以来，（某物质）在谷氨酸和赖氨酸生产中已具有重要的工业价值，并且一直是蛋白质组学和中心碳代谢研究的主题。蛋白质组会根据环境条件、营养可用性和应激源而发生变化。翻译后修饰（PTMs），如磷酸化、甲基化和糖基化，会改变蛋白质的功能和活性，使其能够快速响应环境变化。蛋白质组学技术，如质谱分析和二维凝胶电泳，已能够对蛋白质组进行研究、蛋白质鉴定以及表达水平的定量分析。了解微生物中的蛋白质组和中心碳代谢有助于深入了解其生理学、生态学以及生物技术应用，如生物燃料、药物和工业酶生产。在基因组学和蛋白质组学等多个研究领域，已经进行了多次尝试来创建高效生产菌株以提高生产率。除了氨基酸外，（某物质）还用于生产维生素、核苷酸、有机酸和醇类，从而扩大了其工业应用范围。已经积累了大量信息，但最近的研究集中在蛋白质组和中心碳代谢方面。诸如CRISPR-Cas9等基因工程技术的发展，通过对（某物质）的代谢途径进行精确操纵，提高了生产效率。此外，利用合成生物学技术设计新的代谢途径和开发定制菌株的方法也在逐渐扩展。本综述有望增进对（某物质）及其工业潜力的理解，并帮助研究人员确定研究课题和设计研究方案。
