Pieme Constant Anatole, Tatangmo Jérôme Antony, Simo Gustave, Biapa Nya Prosper Cabral, Ama Moor Vicky Jocelyne, Moukette Moukette Bruno, Tankeu Nzufo Francine, Njinkio Nono Borgia Legrand, Sobngwi Eugene
Department of Biochemistry and Physiological Sciences; Faculty of Medicine and Biomedical Sciences, University of Yaounde I, P.O. Box 1364, Yaounde, Cameroon.
Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
BMC Res Notes. 2017 Mar 29;10(1):141. doi: 10.1186/s13104-017-2463-6.
Studies demonstrate that free radicals are involved in the pathogenesis of diabetic complications. The aim of this study was to determine the implication of total antioxidant capacity (TAC) and some enzymatic and non-enzymatic antioxidants as suitable biomarkers of diabetic complications risk factors.
A total of 90 patients (70 patients with or without diabetic complications +20 normal healthy) were examined by evaluating the level of lipid peroxidation, nitrogen monoxide (NO), fasting blood glucose, glycated haemoglobin (HbA1c), enzymatic and non-enzymatic antioxidants using standard spectrophotometric methods.
The fasting blood glucose and HbA1c levels were respectively 2.05 and 2.32 times higher in the group of patients with diabetes and complications (DPWC) compared to those of healthy persons. A statistically higher level of malondialdehyde (MDA), NO and TAC was observed in a group of patients with diabetes and complications compared to those without complications (DPNC). A significant positive correlation was found between catalase (CAT) and fasting blood glucose while a significant and negative correlation was noted between reduced glutathione (GSH) and fasting blood glucose. Also was noted a significant relationship between HbA1c and other markers of oxidative stress.
The results suggest that the plasma levels of CAT, TAC and reduced glutathione could give information on the risk of developing complications of diabetes, considering that the modification of these biomarkers levels were associated with oxidative stress.
研究表明自由基参与糖尿病并发症的发病机制。本研究的目的是确定总抗氧化能力（TAC）以及一些酶促和非酶促抗氧化剂作为糖尿病并发症危险因素合适生物标志物的意义。
通过使用标准分光光度法评估脂质过氧化水平、一氧化氮（NO）、空腹血糖、糖化血红蛋白（HbA1c）、酶促和非酶促抗氧化剂，对总共90名患者（70名有或无糖尿病并发症的患者 + 20名正常健康者）进行了检查。
与健康人相比，糖尿病及并发症患者组（DPWC）的空腹血糖和HbA1c水平分别高出2.05倍和2.32倍。与无并发症的糖尿病患者组（DPNC）相比，糖尿病及并发症患者组中丙二醛（MDA）、NO和TAC水平在统计学上更高。过氧化氢酶（CAT）与空腹血糖之间存在显著正相关，而还原型谷胱甘肽（GSH）与空腹血糖之间存在显著负相关。此外，还注意到HbA1c与其他氧化应激标志物之间存在显著关系。
结果表明，考虑到这些生物标志物水平的改变与氧化应激相关，CAT、TAC和还原型谷胱甘肽的血浆水平可以提供有关糖尿病并发症发生风险的信息。
