Dept of Paediatrics, Wilhelmina Children's Hospital, University Medical Centre Utrecht, Utrecht, The Netherlands.
Both authors contributed equally.
Eur Respir J. 2017 Mar 29;49(3). doi: 10.1183/13993003.02235-2016. Print 2017 Mar.
Nasopharyngeal and oropharyngeal samples are commonly used to direct therapy for lower respiratory tract infections in non-expectorating infants with cystic fibrosis (CF).We aimed to investigate the concordance between the bacterial community compositions of 25 sets of nasopharyngeal, oropharyngeal and bronchoalveolar lavage (BAL) samples from 17 infants with CF aged ∼5 months (n=13) and ∼12 months (n=12) using conventional culturing and 16S-rRNA sequencing.Clustering analyses demonstrated that BAL microbiota profiles were in general characterised by a mixture of oral and nasopharyngeal bacteria, including commensals like , , and spp. and potential pathogens like , and spp. Within each individual, however, the degree of concordance differed between microbiota of both upper respiratory tract niches and the corresponding BAL.The inconsistent intra-individual concordance between microbiota of the upper and lower respiratory niches suggests that the lungs of infants with CF may have their own microbiome that seems seeded by, but is not identical to, the upper respiratory tract microbiome.
鼻咽和口咽样本常用于指导无痰囊性纤维化 (CF) 婴儿下呼吸道感染的治疗。我们旨在通过常规培养和 16S-rRNA 测序，研究 17 名 5 个月（n=13）和 12 个月（n=12）大的 CF 婴儿的 25 组鼻咽、口咽和支气管肺泡灌洗液（BAL）样本的细菌群落组成之间的一致性。聚类分析表明，BAL 微生物组谱通常以口腔和鼻咽细菌的混合物为特征，包括 、 、 和 属等共生菌和 、 属等潜在病原体。然而，在每个个体中，上呼吸道和相应 BAL 的微生物组之间的一致性程度不同。上呼吸道和下呼吸道微生物组之间不一致的个体内一致性表明，CF 婴儿的肺部可能有自己的微生物组，这些微生物组似乎是由上呼吸道微生物组定植的，但与上呼吸道微生物组不完全相同。
