Zhao Ling, Sun Lv-Hui, Huang Jia-Qiang, Briens Mickael, Qi De-Sheng, Xu Shi-Wen, Lei Xin Gen
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China;
J Nutr. 2017 May;147(5):789-797. doi: 10.3945/jn.116.247338. Epub 2017 Mar 29.
A new organic selenium compound, 2-hydroxy-4-methylselenobutanoic acid (SeO), displayed a greater bioavailability than sodium selenite (SeNa) or seleno-yeast (SeY) in several species. This study sought to determine the regulation of the speciation of selenium, expression of selenogenome and selenocysteine biosynthesis and degradation-related genes, and production of selenoproteins by the 3 forms of selenium in the tissues of broiler chicks. Day-old male chicks ( = 6 cages/diet, 6 chicks/cage) were fed a selenium-deficient, corn and soy-based diet [base diet (BD), 0.05 mg Se/kg] or the BD + SeNa, SeY, or SeO at 0.2 mg Se/kg for 6 wk. Plasma, livers, and pectoral and thigh muscles were collected at weeks 3 and 6 to assay for total selenium, selenomethionine, selenocysteine, redox status, and selected genes, proteins, and enzymes. Although both SeY and SeO produced greater concentrations ( < 0.05) of total selenium (20-172%) and of selenomethionine (≤15-fold) in the liver, pectoral muscle, and thigh than those of SeNa, SeO further raised ( < 0.05) these concentrations by 13-37% and 43-87%, respectively, compared with SeY. Compared with the BD, only SeO enhanced ( 0.05) the mRNA of () and () in the liver and thigh (62-98%) and thioredoxin reductase (TXRND) activity in the pectoral and thigh muscles (20-37%) at week 3. Furthermore, SeO increased ( 0.05) the expression of () , GPX4, SELENOP, and SELENOU relative to the SeNa group by 26-207%, and the expression of , GPX4, and SELENOP relative to the SeY group by 23-55% in various tissues. Compared with SeNa or SeY, SeO demonstrated a unique ability to enrich selenomethionine and total selenium depositions, to induce the early expression of and mRNA and TXRND activity, and to enhance the protein production of GPX4, SELENOP, and SELENOU in the tissues of chicks.
一种新型有机硒化合物，2-羟基-4-甲基硒代丁酸（SeO），在多个物种中表现出比亚硒酸钠（SeNa）或硒酵母（SeY）更高的生物利用度。本研究旨在确定3种形式的硒对肉仔鸡组织中硒形态的调控、硒基因组和硒代半胱氨酸生物合成及降解相关基因的表达以及硒蛋白的产生。1日龄雄性雏鸡（每组6笼，每笼6只鸡）饲喂缺硒的玉米和大豆基础日粮[基础日粮（BD），0.05 mg硒/千克]或添加0.2 mg硒/千克SeNa、SeY或SeO的BD，持续6周。在第3周和第6周采集血浆、肝脏、胸肌和大腿肌肉，检测总硒、硒代蛋氨酸、硒代半胱氨酸、氧化还原状态以及选定的基因、蛋白质和酶。尽管SeY和SeO在肝脏、胸肌和大腿中产生的总硒浓度（P<0.05）（比SeNa高20 - 172%）和硒代蛋氨酸浓度（≤15倍）均高于SeNa，但与SeY相比，SeO分别将这些浓度进一步提高（P<0.05）13 - 37%和43 - 87%。与BD相比，在第3周时，仅SeO增强了（P<0.05）肝脏和大腿中硫氧还蛋白还原酶（TXNRD）的mRNA表达（62 - 98%）以及胸肌和大腿肌肉中硫氧还蛋白还原酶（TXRND）的活性（20 - 37%）。此外，与SeNa组相比，SeO使多种组织中TXNRD、GPX4、SELENOP和SELENOU的表达增加（P<0.05）26 - 207%，与SeY组相比，TXNRD、GPX4和SELENOP的表达增加23 - 55%。与SeNa或SeY相比，SeO具有独特的能力，可富集硒代蛋氨酸和总硒沉积，诱导TXNRD mRNA的早期表达和TXRND活性，并增强雏鸡组织中GPX4、SELENOP和SELENOU的蛋白质产生。
