Gawor Andrzej, Ruszczyńska Anna, Konopka Anna, Wryk Grzegorz, Czauderna Marian, Bulska Ewa
Biological and Chemical Research Centre, Faculty of Chemistry, University of Warsaw, Żwirki i Wigury 101, 02-089 Warsaw, Poland.
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland.
Animals (Basel). 2022 May 31;12(11):1428. doi: 10.3390/ani12111428.
Selenium is an essential nutrient, building twenty five identified selenoproteins in humans known to perform several important biological functions. The small amount of selenium in the earth's crust in certain regions along with the risk of deficiency in organisms have resulted in increasingly popular dietary supplementation in animals, implemented via, e.g., inorganic selenium compounds. Even though selenium is included in selenoproteins in the form of selenocysteine, the dietary effect of selenium may result in the expression of other proteins or genes. Very little is known about the expression effects modulated by selenium. The present study aimed to examine the significance of protein expression in lamb tissues obtained after dietary supplementation with selenium (sodium selenate) and two other feed additives, fish oil and carnosic acid. Label-free mass spectrometry-based proteomic analysis was successfully applied to examine the animal tissues. Protein-protein interaction network analysis of forty differently-expressed proteins following inorganic selenium supplementation indicated two significant clusters which are involved in cell adhesion, heart development, actin filament-based movement, plasma membrane repair, and establishment of organelle localization.
硒是一种必需营养素，在人体内可构成25种已确定的硒蛋白，这些硒蛋白具有多种重要的生物学功能。地壳中某些区域的硒含量较少，加上生物体存在缺乏风险，导致动物通过膳食补充剂（如无机硒化合物）进行补充的做法越来越普遍。尽管硒以硒代半胱氨酸的形式存在于硒蛋白中，但硒的膳食效应可能会导致其他蛋白质或基因的表达。关于硒调节的表达效应，人们了解甚少。本研究旨在探讨在补充硒（硒酸钠）以及另外两种饲料添加剂——鱼油和肌醇六磷酸后，羔羊组织中蛋白质表达的意义。基于无标记质谱的蛋白质组学分析成功应用于检测动物组织。对无机硒补充后40种差异表达蛋白质进行的蛋白质-蛋白质相互作用网络分析表明，有两个显著的聚类，它们参与细胞黏附、心脏发育、基于肌动蛋白丝的运动、质膜修复以及细胞器定位的建立。
