富硒碎米荠增加肉鸡肝脏和胸肌中的硒含量并降低胆固醇浓度。

Selenium-Enriched Cardamine violifolia Increases Selenium and Decreases Cholesterol Concentrations in Liver and Pectoral Muscle of Broilers.

机构信息

Hubei Hongshan Laboratory, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.

Enshi Autonomous Prefecture Academy of Agricultural Sciences, Enshi, Hubei, China.

出版信息

J Nutr. 2022 Sep 6;152(9):2072-2079. doi: 10.1093/jn/nxac141.

DOI:10.1093/jn/nxac141

PMID:35728044

Abstract

BACKGROUND

Supernutrition of selenium (Se) in an effort to produce Se-enriched meat may inadvertently cause lipid accumulation. Se-enriched Cardamine violifolia (SeCv) contains >80% of Se in organic forms.

OBJECTIVES

This study was to determine whether feeding chickens a high dose of SeCv could produce Se-biofortified muscle without altering their lipid metabolism.

METHODS

Day-old male broilers were allocated to 4 groups (6 cages/group and 6 chicks/cage) and were fed either a corn-soy base diet (BD, 0.13-0.15 mg Se/kg), the BD plus 0.5 mg Se/kg as sodium selenite (SeNa) or as SeCv, or the BD plus a low-Se Cardamine violifolia (Cv, 0.20-0.21mg Se/kg). At week 6, concentrations of Se and lipid and expression of selenoprotein and lipid metabolism-related genes were determined in the pectoral muscle and liver.

RESULTS

The 4 diets showed no effects on growth performance of broilers. Compared with the other 3 diets, SeCv elevated (P < 0.05) Se concentrations in the pectoral muscle and liver by 14.4-127% and decreased (P < 0.05) total cholesterol concentrations by 12.5-46.7% and/or triglyceride concentrations by 28.8-31.1% in the pectoral muscle and/or liver, respectively. Meanwhile, SeCv enhanced (P < 0.05) muscular α-linolenic acid (80.0%) and hepatic arachidonic acid (58.3%) concentrations compared with SeNa and BD, respectively. SeCv downregulated (P < 0.05) the cholesterol and triglyceride synthesis-related proteins (sterol regulatory element binding transcription factor 2 and diacylglycerol O-acyltransferase 2) and upregulated (P < 0.05) hydrolysis and β-oxidation of fatty acid-related proteins (lipoprotein lipase, fatty acid binding protein 1, and carnitine palmitoyltransferase 1A), as well as selenoprotein P1 and thioredoxin reductase activity in the pectoral muscle and/or liver compared with SeNa.

CONCLUSIONS

Compared with SeNa, SeCv effectively raised Se and reduced lipids in the liver and muscle of broilers. The effect was mediated through the regulation of the cholesterol and triglyceride biosynthesis and utilization-related genes.

摘要

背景

为了生产富硒肉而过度补充硒（Se）可能会无意中导致脂质积累。富硒碎米荠（SeCv）中超过 80%的硒以有机形式存在。

目的

本研究旨在确定给鸡喂食高剂量的 SeCv 是否可以在不改变其脂质代谢的情况下产生富含硒的肌肉。

方法

1 日龄雄性肉鸡被分配到 4 组（每组 6 个笼子，每个笼子 6 只鸡），分别喂食玉米-大豆基础日粮（BD，0.13-0.15mg Se/kg）、BD 加 0.5mg/kg 亚硒酸钠（SeNa）或 SeCv，或 BD 加低硒碎米荠（Cv，0.20-0.21mg Se/kg）。第 6 周时，测定胸肌和肝脏中硒和脂质的浓度以及硒蛋白和脂质代谢相关基因的表达。

结果

4 种日粮对肉鸡的生长性能没有影响。与其他 3 种日粮相比，SeCv 使胸肌和肝脏中的硒浓度分别提高了 14.4-127%（P<0.05），使胸肌和肝脏中的总胆固醇浓度分别降低了 12.5-46.7%（P<0.05）和/或三酰甘油浓度分别降低了 28.8-31.1%（P<0.05）。同时，与 SeNa 和 BD 相比，SeCv 分别使肌肉中的 α-亚麻酸（80.0%）和肝脏中的花生四烯酸（58.3%）浓度升高（P<0.05）。与 SeNa 相比，SeCv 下调了胸肌和肝脏中胆固醇和三酰甘油合成相关蛋白（固醇调节元件结合转录因子 2 和二酰基甘油 O-酰基转移酶 2）的表达（P<0.05），上调了脂肪水解和β-氧化相关蛋白（脂蛋白脂酶、脂肪酸结合蛋白 1 和肉碱棕榈酰基转移酶 1A）以及胸肌和肝脏中的硒蛋白 P1 和硫氧还蛋白还原酶活性（P<0.05）。