Liu Wan-Sheng, Zhao Yaqi, Lu Chen, Ning Gang, Ma Yun, Diaz Francisco, O'Connor Michael
Department of Animal ScienceCenter for Reproductive Biology and Health (CRBH), College of Agricultural Sciences
Department of Animal ScienceCenter for Reproductive Biology and Health (CRBH), College of Agricultural Sciences.
Reproduction. 2017 Jun;153(6):847-863. doi: 10.1530/REP-17-0013. Epub 2017 Mar 29.
Preferentially expressed antigen in melanoma (PRAME) is a cancer/testis antigen that is predominantly expressed in normal testicular tissues and a variety of tumors. The function of the PRAME family in spermatogenesis remains unknown. This study was designed to characterize the Y-linked PRAME (PRAMEY) protein during spermatogenesis in cattle. We found that PRAMEY is a novel male germ cell-specific, and a germinal granule-associated protein that is expressed in spermatogenic cells during spermatogenesis. The intact PRAMEY protein (58 kDa) was detected in different ages of testes but not in epididymal spermatozoa. A PRAMEY isoform (30 kDa) was highly expressed only in testes after puberty and in epididymal spermatozoa. This isoform interacts with PP1γ2 and is likely the mature protein present in the testes and sperm. Immunofluorescent staining demonstrated that PRAMEY was located predominantly in the acrosome granule of spermatids, and in acrosome and flagellum of spermatozoa. Immunogold electron microscopy further localized the PRAMEY protein complex to the nucleus and several cytoplasmic organelles, including the rough endoplasmic reticulum, some small vesicles, the intermitochondrial cement, the chromatoid body and the centrioles, in spermatogonia, spermatocytes, spermatids and/or spermatozoa. PRAMEY was highly enriched in and structurally associated with the matrix of the acrosomal granule (AG) in round spermatids, and migrated with the expansion of the AG during acrosomal biogenesis. While the function of PRAMEY remains unclear during spermatogenesis, our results suggest that PRAMEY may play an essential role in acrosome biogenesis and spermatogenesis.Free Chinese abstract: A Chinese translation of this abstract is freely available at http://www.reproduction-online.org/content/153/6/847/suppl/DC1.FreeSpanish abstract: A Spanish translation of this abstract is freely available at http://www.reproduction-online.org/content/153/6/847/suppl/DC2.
黑色素瘤中优先表达的抗原（PRAME）是一种癌胚抗原，主要在正常睾丸组织和多种肿瘤中表达。PRAME家族在精子发生中的功能尚不清楚。本研究旨在表征牛精子发生过程中的Y连锁PRAME（PRAMEY）蛋白。我们发现PRAMEY是一种新的雄性生殖细胞特异性且与生发泡相关的蛋白，在精子发生过程中于生精细胞中表达。完整的PRAMEY蛋白（58 kDa）在不同年龄的睾丸中均可检测到，但在附睾精子中未检测到。一种PRAMEY异构体（30 kDa）仅在青春期后的睾丸和附睾精子中高表达。这种异构体与PP1γ2相互作用，可能是睾丸和精子中存在的成熟蛋白。免疫荧光染色表明，PRAMEY主要位于精子细胞的顶体颗粒中，以及精子的顶体和鞭毛中。免疫金电子显微镜进一步将PRAMEY蛋白复合物定位到精原细胞、精母细胞、精子细胞和/或精子的细胞核和几种细胞质细胞器，包括粗面内质网、一些小泡、线粒体间基质、类染色质体和中心粒。PRAMEY在圆形精子细胞的顶体颗粒（AG）基质中高度富集并在结构上与之相关，并在顶体生物发生过程中随着AG的扩张而迁移。虽然PRAMEY在精子发生过程中的功能仍不清楚，但我们的结果表明PRAMEY可能在顶体生物发生和精子发生中起重要作用。免费中文摘要：本摘要的中文翻译可在http://www.reproduction-online.org/content/153/6/847/suppl/DC1免费获取。免费西班牙文摘要：本摘要的西班牙文翻译可在http://www.reproduction-online.org/content/153/6/847/suppl/DC2免费获取。
