Lima-Oliveira Gabriel, Lippi Giuseppe, Salvagno Gian Luca, Picheth Geraldo, Guidi Gian Cesare
Laboratory of Clinical Biochemistry, Department of Life and Reproduction Sciences, University of Verona, Italy; Post-Graduate Program of Pharmaceutical Sciences, Department of Medical Pathology, Federal University of Parana, Curitiba, Parana, Brazil; CAPES Foundation, Ministry of Education of Brazil, Brasília - DF, Brazil; MERCOSUL: Sector Committee of Clinical Analyses and In Vitro Diagnostics - CSM 20, Rio de Janeiro, Brazil.
Laboratory of Clinical Chemistry and Hematology, Academic Hospital of Parma, Parma, Italy.
J Med Biochem. 2015 Jul;34(3):288-294. doi: 10.2478/jomb-2014-0043. Epub 2015 Jul 14.
Diagnostic blood samples collected by phlebotomy are the most common type of biological specimens drawn and sent to laboratory medicine facilities for being analyzed, thus supporting caring physicians in patient diagnosis, follow-up and/or therapeutic monitoring. Phlebotomy, a relatively invasive medical procedure, is indeed critical for the downstream procedures accomplished either in the analytical phase made in the laboratory or in the interpretive process done by the physicians. Diagnosis, management, treatment of patients and ultimately patient safety itself can be compromised by poor phlebotomy quality. We have read with interest a recent article where the authors addressed important aspects of venous blood collection for laboratory medicine analysis. The authors conducted a phlebotomy survey based on the Clinical and Laboratory Standard Institute (CLSI) H03-A6 document (presently replaced by the GP41-A6 document) in three government hospitals in Ethiopia to evaluate 120 professionals (101 non-laboratory professionals vs. 19 laboratory professionals) as regards the venous blood collection practice. The aim of this mini (non-systematic) review is to both take a cue from the above article and from current practices we had already observed in other laboratory settings, and discuss four questionable activities performed by health care professionals during venous blood collection. We refer to: i) diet restriction assessment; ii) puncture site cleansing; iii) timing of tourniquet removal and; iv) mixing specimen with additives.
通过静脉穿刺采集的诊断性血样是送检到检验医学机构进行分析的最常见生物样本类型,从而为负责的医生进行患者诊断、随访和/或治疗监测提供支持。静脉穿刺是一种相对侵入性的医疗操作,对于在实验室进行的分析阶段或医生进行的解读过程中完成的下游程序而言确实至关重要。静脉穿刺质量不佳可能会影响患者的诊断、管理、治疗,最终还可能危及患者安全本身。我们饶有兴趣地阅读了最近一篇文章,作者在文中探讨了用于检验医学分析的静脉血采集的重要方面。作者根据临床和实验室标准协会(CLSI)H03 - A6文件(目前已被GP41 - A6文件取代)在埃塞俄比亚的三家政府医院开展了一项静脉穿刺调查,以评估120名专业人员(101名非实验室专业人员与19名实验室专业人员)的静脉血采集操作情况。本小型(非系统性)综述的目的是借鉴上述文章以及我们在其他实验室环境中已经观察到的当前做法,讨论医护人员在静脉血采集过程中进行的四项有问题的操作。我们指的是:i)饮食限制评估;ii)穿刺部位清洁;iii)松开止血带的时机;iv)将样本与添加剂混合。