Aydin Suleyman, Emre Elif, Ugur Kader, Aydin Mustafa Ata, Sahin İbrahim, Cinar Vedat, Akbulut Taner
Department of Medical Biochemistry and Clinical Biochemistry, Firat Hormones Research Group, School of Medicine, Firat University, Elazig, Turkey.
Department of Anatomy, School of Medicine, Firat University, Elazig, Turkey.
J Int Med Res. 2025 Feb;53(2):3000605251315913. doi: 10.1177/03000605251315913.
The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field.
酶联免疫吸附测定（ELISA）通过使用酶标记的结合物和能产生颜色变化的酶底物来检测抗原 - 抗体相互作用。本综述旨在概述ELISA、其各种类型及其在检测生物体液中代谢物方面的应用。文章讨论了该测定法的历史、其基本原理和程序、常见的ELISA方案以及测量生物体液中肽分子的最准确和可靠的技术。此外，我们强调实现一致、高质量结果的最佳实验室操作规范，并根据我们在该领域30多年的经验概述建立ELISA实验室所需的基本材料。
