Liu Ying, Fu Qi-Zhong, Pu Lin, Meng Qing-Guo, Liu Xian-Feng, Dong Sheng-Fang, Yang Jian-Xun, Lv Guang-Yao
Department of Urology, The Zhongshan Affiliated Hospital of Dalian University, Dalian, 116001, China.
J Med Biochem. 2015 Jul;34(3):338-343. doi: 10.2478/jomb-2014-0036. Epub 2015 Jul 14.
The objective of this study is to detect HMGA2 expression in renal carcinoma to explore its relationship with clinicopathology and its significance in prognosis.
Expressions of HMGA2 mRNA and protein were detected in 50 renal carcinoma specimens, 50 corresponding adjacent normal kidney tissue samples and 40 renal benign tumour specimens via reverse transcription polymerase chain reaction and immunohistochemical assay. Expression analysis was performed along with clinical data analysis.
The relative expression levels of HMGA2 mRNA in renal carcinoma, renal benign tumour tissues and adjacent normal renal tissues were 0.84±0.23, 0.19±0.06 and 0.08±0.04, respectively. HMGA2 protein positive rates were 68.0%, 7.5% and 2.0%, with a significant difference (<0.05). HMGA2 expression was not significantly correlated with gender, age, tumour size and histological type (>0.05), but was significantly correlated with TNM stages and lymph node metastasis (<0.05).
The expressions of HMGA2 gene and protein in renal carcinoma were closely correlated with tumour formation, progression and metastasis. HMGA2 may become a powerful new pathological marker and prognostic factor for renal carcinoma.
本研究旨在检测高迁移率族蛋白A2（HMGA2）在肾癌中的表达情况，以探讨其与临床病理特征的关系及其在预后中的意义。
采用逆转录聚合酶链反应和免疫组织化学分析法，检测50例肾癌标本、50例相应的癌旁正常肾组织标本及40例肾良性肿瘤标本中HMGA2 mRNA和蛋白的表达情况，并结合临床资料进行表达分析。
HMGA2 mRNA在肾癌组织、肾良性肿瘤组织及癌旁正常肾组织中的相对表达水平分别为0.84±0.23、0.19±0.06和0.08±0.04。HMGA2蛋白阳性率分别为68.0%、7.5%和2.0%，差异有统计学意义（<0.05）。HMGA2表达与性别、年龄、肿瘤大小及组织学类型无明显相关性（>0.05），但与TNM分期及淋巴结转移显著相关（<0.05）。
HMGA2基因和蛋白在肾癌中的表达与肿瘤的发生、发展及转移密切相关。HMGA2可能成为一种有力的肾癌新病理标志物和预后因素。
