Department of Urology and Hubei Key Laboratory of Kidney Disease Pathogenesis and Interventio, Huangshi Central Hospital, Edong Healthcare Group, Affiliated Hospital of Hubei Polytechnic University, Huangshi, China.
J Clin Lab Anal. 2022 Jun;36(6):e24422. doi: 10.1002/jcla.24422. Epub 2022 Apr 25.
Long noncoding RNAs (LncRNAs) plays a vital role in tumorigenesis and development. The molecular mechanism of SNHG1 in renal cell carcinoma (RCC) has not been illustrated. The aim of this research was to explore the expression and function of LncRNA SNHG1 in RCC.
The expression of SNHG1 in clinical tissues and RCC cell lines was detected. Luciferase reporter assay was performed to verify the correlation between SNHG1, miR-103a, and HMGA2. CCK-8 assay was performed to examine cell viability. Cell apoptosis was analyzed using flow cytometry. Cell invasion capacity was determined by Transwell assays. The protein level of HMGA2 was analyzed by Western blotting.
The expression of SNHG1 markedly increased in RCC tissues and cell lines. Subsequent studies identified SNHG1 as a miRNA sponge for miR-103a. In addition, SNHG1 knockdown and miR-103a overexpression significantly inhibited progression of RCC. miR-103a also regulated HMGA2 levels.
Our findings showed that SNHG1 was upregulated in RCC cells and tissues. SNHG1 promoted the malignant characteristics of RCC cells. Its regulatory effect may be regulation of HMGA2 by sponging miR-103a. Therefore, Our study facilitates the understanding of SNHG1 function in RCC.
长链非编码 RNA（LncRNA）在肿瘤发生和发展中起着至关重要的作用。SNHG1 在肾细胞癌（RCC）中的分子机制尚未阐明。本研究旨在探讨 LncRNA SNHG1 在 RCC 中的表达和功能。
检测 SNHG1 在临床组织和 RCC 细胞系中的表达。荧光素酶报告实验验证 SNHG1、miR-103a 和 HMGA2 之间的相关性。CCK-8 法检测细胞活力。流式细胞术分析细胞凋亡。Transwell 测定分析细胞侵袭能力。Western blot 分析 HMGA2 蛋白水平。
SNHG1 在 RCC 组织和细胞系中表达显著增加。进一步的研究表明，SNHG1 是 miR-103a 的 miRNA 海绵。此外，SNHG1 敲低和 miR-103a 过表达显著抑制了 RCC 的进展。miR-103a 还调节 HMGA2 水平。
我们的研究结果表明，SNHG1 在 RCC 细胞和组织中上调。SNHG1 促进了 RCC 细胞的恶性特征。其调节作用可能是通过海绵吸附 miR-103a 调节 HMGA2。因此，本研究有助于理解 SNHG1 在 RCC 中的功能。
