Zhang Yifei, Song Xiaojing, Wang Xixun, Hu Jinchen, Jiang Lixin
Department of Thyroid Surgery in Gastrointestinal Surgery, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai City, Shandong Province, P. R. China.
Library, the Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai City, Shandong Province, P. R. China.
J Med Biochem. 2016 Apr;35(2):137-143. doi: 10.1515/jomb-2015-0016. Epub 2016 May 9.
Gastric cancer (GC) is one of the most common cancers in the world; however, chemoresistance greatly decreases the efficacy of therapy in gastric cancer. Long noncoding RNAs (IncRNAs) participate in a variety of biological processes, and we hypothesize that lncRNA HULC regulates the multidrug resistance in GC treatment.
We obtained GC tissue samples from 42 GC patients and detected the expression level of HULC in the plasma and tissues via qRT-PCR. The relationship between HULC expression and survival rate was confirmed by Kaplan-Meier survival analysis. We verified the expression of HULC in GC cell lines via qRT-PCR, and the function of HULC was detected via flow cytometry assay and CCK-8 assay.
HULC was highly expressed in the plasma and tissues of the GC patients compared with controls, with HULC high expression indicating lower survival rate. HULC knockdown enhanced cisplatin-induced apoptosis in GC cells.
Our results suggest that silencing lncRNA HULC could enhance chemotherapy induced apoptosis in GC cells, which could provide a novel approach for therapeutic strategies.
胃癌（GC）是世界上最常见的癌症之一；然而，化疗耐药性大大降低了胃癌治疗的疗效。长链非编码RNA（lncRNAs）参与多种生物学过程，我们推测lncRNA HULC在胃癌治疗中调节多药耐药性。
我们从42例胃癌患者中获取胃癌组织样本，通过qRT-PCR检测血浆和组织中HULC的表达水平。通过Kaplan-Meier生存分析确定HULC表达与生存率之间的关系。我们通过qRT-PCR验证了HULC在胃癌细胞系中的表达，并通过流式细胞术检测和CCK-8检测来检测HULC的功能。
与对照组相比，HULC在胃癌患者的血浆和组织中高表达，HULC高表达表明生存率较低。HULC敲低增强了顺铂诱导的胃癌细胞凋亡。
我们的结果表明，沉默lncRNA HULC可以增强化疗诱导的胃癌细胞凋亡，这可能为治疗策略提供一种新方法。
