Cook N, Nahorski S R, Jagger C, Barnett D B
Department of Pharmacology, Leicester Royal Infirmary, UK.
Naunyn Schmiedebergs Arch Pharmacol. 1988 Feb;337(2):238-40. doi: 10.1007/BF00169254.
The presence of beta 2 adrenoceptors on the human platelet is well established although the coupling of this receptor to adenylate cyclase is disputed. In this study of highly purified platelets isoprenaline did not stimulate whole platelet cyclic AMP alone or in the presence of forskolin. Minimal contamination of the purified platelets (approximately 0.05%) with mononuclear white blood cells may lead to the spurious appearance of isoprenaline stimulated cAMP. These results confirm our previous findings of lack of coupling of the human platelet beta 2 adrenoceptor to adenylate cyclase and indicate the importance of purity of preparation for platelet biochemical studies.
人类血小板上β2肾上腺素能受体的存在已得到充分证实，尽管该受体与腺苷酸环化酶的偶联存在争议。在这项对高度纯化血小板的研究中，异丙肾上腺素单独或在福斯高林存在的情况下均未刺激全血小板环磷酸腺苷（cAMP）。纯化血小板中单核白细胞的最低污染率（约0.05%）可能导致异丙肾上腺素刺激的cAMP出现假阳性。这些结果证实了我们之前关于人类血小板β2肾上腺素能受体与腺苷酸环化酶缺乏偶联的发现，并表明制备纯度对于血小板生化研究的重要性。
