Liu Rui, Ji Ping, Liu Bin, Qiao Haishi, Wang Xia, Zhou Likun, Deng Ting, Ba Yi
Department of Gastrointestinal Oncology, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China.
Jiangsu Key Laboratory of Molecular Medicine, Nanjing University Medical School, Nanjing, Jiangsu 210093, P.R. China.
Oncol Lett. 2017 Feb;13(2):1024-1030. doi: 10.3892/ol.2016.5495. Epub 2016 Dec 14.
Epidemiological and experimental evidence suggests that dietary flavonoids, including apigenin, have anticancer roles. Apigenin has been reported to elevate p53, a critical molecule in the induction of apoptosis. The present study aimed to investigate whether apigenin, a dietary flavonoid, improves the cytotoxic effect of cisplatin in a cancer cell culture system, and to elucidate the mechanism of this effect. Multiple tumor cell types were treated with apigenin, cisplatin or both drugs. Cell viability was evaluated, and the cytotoxic effect was determined biochemically and microscopically. Treatment with apigenin increased cisplatin-induced DNA damage and the apoptosis of tumor cells in a p53-dependent manner. Apigenin, when used with cisplatin, inhibited cell proliferation and promoted mitogen-activated protein kinase activation and subsequent p53 phosphorylation, leading to p53 accumulation and upregulation of proapoptotic proteins. Cisplatin is one of the most commonly used chemotherapeutic drugs for malignant tumors, but resistance to this drug occurs. The current results therefore demonstrate that dietary flavonoids may diminish the resistance of cancers to cisplatin.
流行病学和实验证据表明，包括芹菜素在内的膳食类黄酮具有抗癌作用。据报道，芹菜素可提高p53水平，p53是诱导细胞凋亡的关键分子。本研究旨在探讨膳食类黄酮芹菜素是否能增强顺铂在癌细胞培养系统中的细胞毒性作用，并阐明其作用机制。用芹菜素、顺铂或两种药物处理多种肿瘤细胞类型。评估细胞活力，并通过生化和显微镜方法确定细胞毒性作用。芹菜素处理以p53依赖的方式增加了顺铂诱导的DNA损伤和肿瘤细胞凋亡。芹菜素与顺铂联合使用时，可抑制细胞增殖，促进丝裂原活化蛋白激酶激活及随后的p53磷酸化，导致p53积累和促凋亡蛋白上调。顺铂是治疗恶性肿瘤最常用的化疗药物之一，但会出现对该药物的耐药性。因此，目前的结果表明，膳食类黄酮可能会降低癌症对顺铂的耐药性。
