Thapar Upasna, Demple Bruce
Department of Pharmacological Sciences, Stony Brook University, School of Medicine, Stony Brook, NY, USA.
F1000Res. 2017 Mar 16;6:279. doi: 10.12688/f1000research.10538.1. eCollection 2017.
Since the discovery of the base excision repair (BER) system for DNA more than 40 years ago, new branches of the pathway have been revealed at the biochemical level by studies. Largely for technical reasons, however, the confirmation of these subpathways has been elusive. We review methods that have been used to explore BER in mammalian cells, indicate where there are important knowledge gaps to fill, and suggest a way to address them.
自40多年前发现DNA碱基切除修复（BER）系统以来，该途径的新分支已在生化水平上通过研究得以揭示。然而，很大程度上由于技术原因，这些亚途径的确认一直难以实现。我们回顾了用于探索哺乳动物细胞中BER的方法，指出了存在的重要知识空白，并提出了填补这些空白的方法。
