Salimi Saeedeh, Shahrakipour Mahnaz, Hajizadeh Azam, Mokhtari Mojgan, Mousavi Mahdieh, Teimoori Batool, Yaghmaei Minoo
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran; Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran.
Department of Biostatistics and Epidemiology, School of Public Health, Zahedan University of Medical Sciences, Zahedan, Sistan and Baluchestan 9816743175, Iran.
Biomed Rep. 2017 Feb;6(2):237-241. doi: 10.3892/br.2016.830. Epub 2016 Dec 21.
Uterine leiomyoma (UL) is the most common benign tumor causing considerable morbidity during the reproductive years in women. Cyclin D1 (CCND1) is a cell cycle regulatory protein that is required for the G1 phase, and increased expression levels of this protein may affect tumorigenesis. The present study aimed to assess the possible effect of the G870A polymorphism on UL susceptibility. A total of 154 women with UL and 197 healthy women who were age-, body mass index (BMI)- and ethnicity-matched were genotyped for the G870A (rs9344) polymorphism using the polymerase chain reaction-restriction fragment length polymorphism method. The effects of G870A transition on the structure of mRNA and proteins of CCND1 was evaluated using bioinformatics tools. The frequency of the 870AA genotype was significantly higher in women with UL compared with the control subjects, and the risk of UL was 1.4-fold higher in women with the AA genotype when compared with the GG genotype before and after adjusting for age, BMI, and ethnicity [odds ratio (OR), 1.4; 95% confidence interval (CI), 1.1-2 (P=0.02)]. The frequency of 870GA genotype was not significantly different between the two groups. The frequency of the 870A allele was significantly higher in the women with UL when compared with the control subjects (57 vs. 48%; P=0.02). The analysis revealed that the G870A transition may fundamentally alter the structure of the CCND1-mRNA. Thus, the CCND1 870AA genotype was associated with UL susceptibility in a sample of women from the southeast of Iran.
子宫肌瘤（UL）是女性生殖年龄期间最常见的良性肿瘤，会引发相当高的发病率。细胞周期蛋白D1（CCND1）是一种细胞周期调节蛋白，在G1期发挥作用，该蛋白表达水平升高可能影响肿瘤发生。本研究旨在评估G870A多态性对UL易感性的可能影响。采用聚合酶链反应-限制性片段长度多态性方法，对154例UL患者和197例年龄、体重指数（BMI）和种族相匹配的健康女性进行G870A（rs9344）多态性基因分型。使用生物信息学工具评估G870A转换对CCND1的mRNA和蛋白质结构的影响。与对照组相比，UL女性中870AA基因型的频率显著更高，在调整年龄、BMI和种族后，AA基因型女性患UL的风险比GG基因型高1.4倍[比值比（OR），1.4；95%置信区间（CI），1.1 - 2（P = 0.02）]。两组之间870GA基因型的频率无显著差异。与对照组相比，UL女性中870A等位基因的频率显著更高（57%对48%；P = 0.02）。分析显示G870A转换可能从根本上改变CCND1 - mRNA的结构。因此，在来自伊朗东南部的女性样本中，CCND1 870AA基因型与UL易感性相关。
