Salimi Saeedeh, Hajizadeh Azam, Yaghmaei Minoo, Rezaie Sodabeh, Shahrakypour Mahnaz, Teimoori Batool, Parache Mahboube, Naghavi Anoosh, Mokhtari Mojgan
Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Tumour Biol. 2016 Sep;37(9):12497-12502. doi: 10.1007/s13277-016-5078-y. Epub 2016 Jun 23.
Uterine leiomyoma (UL) is a monoclonal tumor which arises from uninhibited proliferation of a single myometrial cell; therefore, the imbalance in cell cycle regulation could be a key event in its development. In the present study, we aimed to assess the association of p21 gene polymorphisms and UL. Genomic DNA was extracted from blood samples of 154 women with UL and 197 age-, BMI-, and ethnically matched controls. p21 C98A (rs1801270) and C70T (rs1059234) polymorphism genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The CA genotype of p21 C98A polymorphism was significantly higher in UL women (28 %) compared to the controls (18 %), and the UL risk was 1.8-fold greater in women with CA genotype compared to CC genotype before and after adjusting for age, BMI, and ethnicity (OR, 1.8 [95 % CI, 1.1 to 3]; P = 0.02). There was no association between the AA genotype of p21 C98A polymorphism and UL. Moreover, the frequency of p21 98A allele was significantly higher in the UL women compared to controls (17 vs. 12 %, p = 0.04). The p21 C70T polymorphism did not correlate with UL before and after adjusting for age, BMI, and ethnicity. There was no difference in haplotype frequency of p21 C70T and C98A polymorphisms between UL patients and the controls. CA genotype of p21 C98A polymorphism may be a risk factor for UL susceptibility; however, p21 C70T polymorphism did not associate with UL.
子宫平滑肌瘤（UL）是一种单克隆肿瘤，由单个子宫肌层细胞不受抑制地增殖产生；因此，细胞周期调控失衡可能是其发生发展的关键事件。在本研究中，我们旨在评估p21基因多态性与UL的关联。从154例UL患者及197例年龄、体重指数（BMI）和种族相匹配的对照者的血液样本中提取基因组DNA。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析确定p21 C98A（rs1801270）和C70T（rs1059234）多态性基因型。与对照组（18%）相比，UL患者中p21 C98A多态性的CA基因型显著更高（28%），在调整年龄、BMI和种族后，CA基因型女性患UL的风险比CC基因型女性高1.8倍（比值比[OR]，1.8[95%置信区间，1.1至3]；P = 0.02）。p21 C98A多态性的AA基因型与UL之间无关联。此外，与对照组相比，UL患者中p21 98A等位基因的频率显著更高（17%对12%，P = 0.04）。在调整年龄、BMI和种族前后，p21 C70T多态性与UL均无相关性。UL患者与对照组之间p21 C70T和C98A多态性的单倍型频率无差异。p21 C98A多态性的CA基因型可能是UL易感性的一个危险因素；然而，p21 C70T多态性与UL无关。
