Maeda Kazuyuki, Nakajima Yuichi, Tanahashi Yoshikazu, Kitou Yoshiyuki, Miwa Akihiro, Kanamaru Kyoko, Kobayashi Tetsuo, Nishiuchi Takumi, Kimura Makoto
Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
Division of Functional Genomics, Advanced Science Research Centre, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa, 920-0934, Japan.
Arch Microbiol. 2017 Aug;199(6):945-952. doi: 10.1007/s00203-017-1364-3. Epub 2017 Mar 29.
Fusarium graminearum produces trichothecene mycotoxins under certain nutritional conditions. When L-Thr and its analogue L-allo-threonine were added to brown rice flour solid medium before inoculation, trichothecene production after 4 days of incubation was suppressed. A time-course analysis of gene expression demonstrated that L-Thr suppressed transcription of Tri6, a trichothecene master regulator gene, and a terpene cyclase Tri5 gene. Regulation of trichothecene biosynthesis by altering major primary metabolic processes may open up the possibility to develop safe chemicals for the reduction of mycotoxin contamination might be developed.
禾谷镰刀菌在特定营养条件下产生单端孢霉烯族霉菌毒素。在接种前将L-苏氨酸及其类似物L-别苏氨酸添加到糙米粉固体培养基中，培养4天后单端孢霉烯族霉菌毒素的产生受到抑制。基因表达的时间进程分析表明，L-苏氨酸抑制了单端孢霉烯族霉菌毒素主要调控基因Tri6和萜烯环化酶Tri5基因的转录。通过改变主要初级代谢过程来调控单端孢霉烯族霉菌毒素的生物合成，可能为开发减少霉菌毒素污染的安全化学品开辟道路。
