HhaII核酸内切酶-DNA共晶体的初步X射线衍射分析。

Preliminary X-ray diffraction analysis of HhaII endonuclease-DNA cocrystals.

作者信息

Chandrasegaran S, Smith H O, Amzel M L, Ysern X

机构信息

Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

出版信息

Proteins. 1986 Nov;1(3):263-6. doi: 10.1002/prot.340010309.

DOI:10.1002/prot.340010309

PMID:2835764

Abstract

HhaII restriction endonuclease purified from an overproducing recombinant E. coli clone has been cocrystallized with a heptanucleotide duplex, d-GGAGTCC:GGACTCC. The cocrystals are monoclonic and belong to the space group C2. The unit cell dimensions are a = 199.0 +/- 1.0 A, b = 100.0 +/- 0.5 A, c = 80.3 +/- 0.4 A, and beta = 101.0 +/- 1.0 degrees. There appear to be two dimers per asymmetric unit and the crystals diffract to 4-A resolution.

摘要

从一个过量表达的重组大肠杆菌克隆中纯化得到的HhaII限制性内切酶已与一个七核苷酸双链体d-GGAGTCC:GGACTCC共结晶。这些共晶体是单克隆的，属于空间群C2。晶胞参数为a = 199.0 +/- 1.0 Å，b = 100.0 +/- 0.5 Å，c = 80.3 +/- 0.4 Å，β = 101.0 +/- 1.0°。每个不对称单元似乎有两个二聚体，并且这些晶体的衍射分辨率达到4 Å。