Laboratory for Chemistry and Physics of Interfaces, Department of Microsystems Engineering, University of Freiburg , Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
ACS Appl Mater Interfaces. 2017 Apr 12;9(14):12165-12170. doi: 10.1021/acsami.7b01232. Epub 2017 Apr 3.
A novel, highly efficient method for the preparation of functional, microstructured and surface-attached cryogels is described. Photoinduced C,H-insertion reactions are used to generate cryogels in a single, rapid photo-cross-linking process. To this end, solutions containing both a photoreactive copolymer and the (bio)molecules to be immobilized are placed on a polymeric substrate followed by freezing and a short UV exposure. This strategy combines photolithography and cryogel formation allowing for a simultaneous generation and (bio)functionalization of cryogels in a single reaction step. To demonstrate the potential of the generated materials for bioanalytical applications, we successfully prepared DNA and protein cryogel microarrays.
本文描述了一种新颖、高效的制备功能化、微结构化和表面附着的冷冻凝胶的方法。光诱导的 C，H-插入反应被用于在单一的快速光交联过程中生成冷冻凝胶。为此，将含有光反应性共聚物和待固定的（生物）分子的溶液放置在聚合物基底上，然后进行冷冻和短时间的紫外光照射。该策略结合了光刻技术和冷冻凝胶形成，允许在单个反应步骤中同时生成和（生物）功能化冷冻凝胶。为了展示所生成材料在生物分析应用中的潜力，我们成功地制备了 DNA 和蛋白质冷冻凝胶微阵列。
