Chemistry and Physics of Interfaces, Department of Microsystems Engineering - IMTEK, University of Freiburg, 79110 Freiburg, Germany.
ACS Appl Mater Interfaces. 2022 Jun 8;14(22):25147-25154. doi: 10.1021/acsami.2c04361. Epub 2022 May 26.
Current developments in precision medicine require the simultaneous detection of an increasing number of biomarkers in heterogeneous, complex solutions, such as blood samples. To meet this need, immunoassays on barcoded hydrogel beads have been proposed, although the encoding and decoding of these barcodes is usually complex and/or resource-intensive. Herein, an efficient method for the fabrication of barcoded, functionalized hydrogel beads is presented. The hydrogel beads are generated using droplet-based microfluidics in combination with photochemically induced C-H insertion reactions, allowing photo-crosslinking, (bio-) functionalization, and barcode integration to be performed in a single step. The generated functionalized beads carry single-color barcodes consisting of green-fluorescent particles of different sizes and concentrations, allowing simple and simultaneous readout with a standard plate reader. As a test example, the performance of barcoded hydrogel beads (3 × 3 matrix) functionalized with capture molecules of interest (e.g., antigens) is investigated for the detection of Lyme-disease-specific antibodies in patient sera. The described barcoding strategy for hydrogel beads does not interfere with the bioanalytical process and captivates by its simplicity and versatility, making it an attractive candidate for multiplex bioanalytical processes.
精准医学的当前发展需要在异质、复杂的溶液（如血液样本）中同时检测越来越多的生物标志物。为了满足这一需求，已经提出了基于带有条码的水凝胶珠的免疫测定法，尽管这些条码的编码和解码通常较为复杂和/或资源密集。在此，提出了一种用于制备带有条码的功能化水凝胶珠的有效方法。水凝胶珠是使用基于液滴的微流控技术结合光化学诱导的 C-H 插入反应生成的，允许在单个步骤中进行光交联、（生物）功能化和条码集成。生成的功能化珠带有由不同大小和浓度的绿色荧光颗粒组成的单彩色条码，允许使用标准平板读数器进行简单且同时的读出。作为测试示例，研究了带有感兴趣的捕获分子（例如抗原）的条码化水凝胶珠（3×3 矩阵）用于检测患者血清中莱姆病特异性抗体的性能。所描述的水凝胶珠的条码策略不会干扰生物分析过程，其简单性和多功能性使其成为多路生物分析过程的有吸引力的候选者。
