Sivakumar A, Kumar Subodh, Yathish H M, Mishra Chinmoy, Modi Rajendra Prasad, Chaudhary Rajni, Khan Subuhi, Sivamani B, Ghosh S K, Sarkar Mihir
a Division of Animal Genetics, Indian Veterinary Research Institute , Izatnagar , India.
b Department of Animal Genetics and Breeding, Veterinary College , Bidar , India.
Anim Biotechnol. 2018 Jan 2;29(1):34-40. doi: 10.1080/10495398.2017.1294597. Epub 2017 Mar 30.
122 randomly selected Vrindavani cattle were studied to detect polymorphism in four fragments of the CatSper2 gene that were comprised of exon 2, 4, 5, and 6 with flanking regions. Using PCR-SSCP and sequencing analysis, three SNPs (T157C, C273A, and A274C) in the first fragment, one SNP (C30G) in the second fragment, and two SNPs (T86G and T292C) in the fourth fragment were identified. The third fragment did not reveal any polymorphism. The SNPs were used for construction of haplotypes and three haplotypes were found. The least square analysis of variance revealed a significant (P < 0.01) effect of haplotype on all three motility parameters. The haplotype II and III were nonsignificantly different from each other while being significantly (P < 0.01) different from haplotype I. The nonsignificant difference of haplotype II with III can lead to a hypothesis that T>G or C>T SNPs may not play a role in sperm motility. However, when the comparison was made between haplotype I and II, it can be inferred that C>T SNP may have a role in sperm motility, as haplotype II has better motility parameters. Expression profiling of Catper2 gene revealed nonsignificant down regulation of CatSper2 gene in poor motility sperm compared to good motility sperm.
对122头随机挑选的弗林达瓦尼牛进行研究，以检测CatSper2基因四个片段的多态性，这些片段由外显子2、4、5和6及其侧翼区域组成。通过PCR-SSCP和测序分析，在第一个片段中鉴定出3个单核苷酸多态性（SNP）（T157C、C273A和A274C），在第二个片段中鉴定出1个SNP（C30G），在第四个片段中鉴定出2个SNP（T86G和T292C）。第三个片段未发现任何多态性。利用这些SNP构建单倍型，共发现3种单倍型。方差的最小二乘分析显示，单倍型对所有三个活力参数均有显著（P < 0.01）影响。单倍型II和III之间差异不显著，但与单倍型I有显著（P < 0.01）差异。单倍型II与III之间的不显著差异可能导致这样一个假设，即T>G或C>T的SNP可能对精子活力没有影响。然而，当比较单倍型I和II时，可以推断C>T的SNP可能对精子活力有影响，因为单倍型II具有更好的活力参数。Catper2基因的表达谱显示，与活力良好的精子相比，活力较差的精子中CatSper2基因的下调不显著。
