Hirenallur Maheshwarappa Yathish, Kumar Subodh, Chaudhary Rajni, Mishra Chinmoy, Ayyar Sivakumar, Kumar Amit, Chauhan Anuj, Ghosh Subrata Kumar, Panigrahi Manjit, Mitra Abhijit
Division of Animal Genetics, ICAR-Indian Veterinary Research Institute (IVRI), Bareilly, India.
Reprod Domest Anim. 2019 Feb;54(2):365-372. doi: 10.1111/rda.13369. Epub 2018 Nov 19.
Transition proteins (TNPs) are essential in chromatin condensation during spermiogenesis, and hence, they are the candidate genes for identifying sperm motility markers. Coding and in silico predicted promoter regions of these genes were investigated in crossbred and purebred cattle, and also, their mRNA quantification was done to explore its use as a diagnostic tool of infertility. PCR-SSCP analysis revealed two band patterns in fragment III of TNP1 and fragment II of TNP2 gene. Sequence analysis revealed a deletion of "G" nucleotide in 3'UTR region of TNP1 and C>T SNP in intronic region of TNP2 gene. Least square analysis of variance did not reveal any significant influence of nucleotide deletion on any sperm motility parameters in both crossbred and purebred cattle. However, C>T SNP had a significant effect on initial progressive motility (p < 0.05) in purebred cattle and post-thaw motility in overall cattle population. RT-qPCR analysis did not reveal any significant variation in TNP1 and TNP2 gene expression among poorly motile and good quality spermatozoa of Vrindavani bulls.
过渡蛋白（TNPs）在精子发生过程中的染色质浓缩中起着至关重要的作用，因此，它们是识别精子活力标志物的候选基因。对这些基因的编码区和通过计算机预测的启动子区域在杂交牛和纯种牛中进行了研究，并且还对它们的mRNA进行了定量分析，以探索其作为不孕症诊断工具的用途。PCR-SSCP分析在TNP1基因的片段III和TNP2基因的片段II中揭示了两种条带模式。序列分析显示TNP1基因3'UTR区域缺失“G”核苷酸，TNP2基因内含子区域存在C>T单核苷酸多态性。方差的最小二乘分析未显示核苷酸缺失对杂交牛和纯种牛的任何精子活力参数有任何显著影响。然而，C>T单核苷酸多态性对纯种牛的初始渐进性活力（p < 0.05）和总体牛群解冻后的活力有显著影响。RT-qPCR分析未显示弗林达瓦尼公牛中活力差和质量好的精子之间TNP1和TNP2基因表达有任何显著差异。
