Girbau Cecilia, Martinez-Malaxetxebarria Irati, Muruaga Gorka, Carmona Sandra, Alonso Rodrigo, Fernandez-Astorga Aurora
Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), C/Paseo de la Universidad 7, 01006, Vitoria-Gasteiz, Spain (ORCID: http://orcid.org/0000-0002-6576-6707 [I.M.-M.]).
J Food Prot. 2017 May 1;80(5):758-762. doi: 10.4315/0362-028X.JFP-16-505.
The transmission of Arcobacter butzleri, an emerging food- and waterborne pathogen, is possibly favored by its ability to adhere to abiotic surfaces. In this study, we assessed the biofilm formation ability of 42 A. butzleri isolates recovered from different food products. Overall, nine isolates (21.4%) were able to adhere to polystyrene. Among them, a chicken-derived isolate was classified as strongly adherent. Based on the chi-square test, no relation was found between the adhesive abilities of the isolates and their source (P > 0.05). An aerobic atmosphere enhanced the adhesion ability of the majority of the adherent isolates (66.7%), because when tested in microaerobic conditions, a t test indicated that only three isolates increased their biofilm formation ability significantly (P < 0.05). In addition, seven (77.8%) of these nine isolates were able to adhere to glass surfaces, and viable cells were recovered from all the stainless steel coupons tested. Therefore, our results confirm the biofilm formation ability of A. butzleri, which may be influenced by the incubation atmosphere and the abiotic surface.
布氏弓形杆菌是一种新出现的食源和水源性病原体，其附着于非生物表面的能力可能有利于其传播。在本研究中，我们评估了从不同食品中分离出的42株布氏弓形杆菌的生物膜形成能力。总体而言，9株分离株（21.4%）能够附着于聚苯乙烯。其中，一株源自鸡肉的分离株被归类为强附着型。基于卡方检验，未发现分离株的附着能力与其来源之间存在关联（P>0.05）。需氧环境增强了大多数附着型分离株（66.7%）的附着能力，因为在微需氧条件下进行测试时，t检验表明只有3株分离株的生物膜形成能力显著增加（P<0.05）。此外，这9株分离株中有7株（77.8%）能够附着于玻璃表面，并且从所有测试的不锈钢试片上都回收了活细胞。因此，我们的结果证实了布氏弓形杆菌的生物膜形成能力，这可能受培养环境和非生物表面的影响。
