CICS-UBI-Health Sciences Research Centre, University of Beira Interior, Avenida Infante D. Henrique, 6200-506 Covilhã, Portugal.
Int J Food Microbiol. 2013 Mar 1;162(1):82-8. doi: 10.1016/j.ijfoodmicro.2013.01.003. Epub 2013 Jan 11.
The genus Arcobacter is an emerging pathogen associated with several clinical symptoms. This genus is widely distributed and has been isolated from environmental, animal, food and human samples, where poultry is considered the major source. In this study, forty three Arcobacter butzleri strains isolated from poultry and environment of a Portuguese slaughterhouse, were characterized by pulsed field gel electrophoresis (PFGE) and assessed for antimicrobial susceptibility and ability to form biofilms. PFGE patterns obtained using restriction enzymes SmaI and SacII revealed high genetic diversity, with 32 distinct PFGE patterns. Most of A. butzleri isolates presented multiple antimicrobial resistance, exhibiting four different resistance profiles. All 43 isolates were susceptible to gentamicin and 2.3% were resistant to chloramphenicol, in contrast to twenty four (55.8%) that were resistant to ciprofloxacin. Among 36 selected isolates, 26 strains presented biofilm-forming ability, which was dependent on the atmosphere and initial inoculum density. Overall, the results showed that A. butzleri displays a high genetic diversity, and presents resistance to several antibiotics, which together with its biofilm formation ability may represent a potential hazard for foodborne infections and a considerable risk for human health.
弧菌属是一种与多种临床症状相关的新兴病原体。该属广泛分布，已从环境、动物、食品和人类样本中分离出来，其中家禽被认为是主要来源。在这项研究中，从葡萄牙一家屠宰场的家禽和环境中分离出的 43 株弯曲杆菌，但泽勒被脉冲场凝胶电泳（PFGE）进行了特征描述，并评估了它们对抗菌药物的敏感性和形成生物膜的能力。使用 SmaI 和 SacII 两种限制酶获得的 PFGE 图谱显示出高度的遗传多样性，有 32 种不同的 PFGE 图谱。大多数弯曲杆菌但泽勒分离株表现出多种抗药性，表现出四种不同的耐药谱。所有 43 株分离株均对庆大霉素敏感，而对氯霉素的耐药率为 2.3%，而对环丙沙星的耐药率为 24 株（55.8%）。在 36 株选定的分离株中，有 26 株具有形成生物膜的能力，这取决于大气和初始接种密度。总的来说，结果表明弯曲杆菌但泽勒显示出高度的遗传多样性，并对几种抗生素表现出耐药性，加上其形成生物膜的能力，可能对食源性病原体感染构成潜在威胁，对人类健康构成重大风险。
